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Product details for Fas (CD95) Human antibody
Fas (CD95) Human antibody
Overview
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| Antigen: | Fas (CD95) |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS) |
| Conjugate: | Biotin |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 149,83 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN158205 |
| Availability: | Ships within 7 to 10 days |
Additional Information: Fas (CD95) Human antibody
| Antigen | Fas (CD95) |
| Immunogen | CD95 transfected L cells |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Isotype | Mouse IgG1, kappa »Matching secondary antibodies |
| Description | CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis, and has been shown to play a role in the maintenance of peripheral tolerance. The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Distribution: T and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes Function: Mediates apoptosis Synonyms: Fas, APO-1, TNFRSF6 Structure: TNFR superfamily, type I transmembrane protein, 45 kD |
| Clone | DX2 |
| Host | Mouse |
Application Details: Fas (CD95) Human antibody
| Application | Flow Cytometry (FACS) |
| Application Notes | Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis(DX2 antibody is required to be cross-linked for effective induction of apoptosis.) , and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158216). Note: EOS9.1 antibody (cat. No.ABIN158223/305704) can induce apoptosis without cross-linking process. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | Biotin |
| Purification | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C. Do not freeze. |
| Research Area | CD Antigens |
| Restrictions | For Research Use only |
Publications: Fas (CD95) Human antibody
| Publications |
Krammer, Dhein, Walczak et al.: "The role of APO-1-mediated apoptosis in the immune system." In: Immunological reviews , Vol. 142, pp. 175-91, 1995/01 (PubMed) Nagata, Golstein: "The Fas death factor." In: Science (New York, N.Y.) , Vol. 267, Issue 5203, pp. 1449-56, 1995/01 (PubMed) Cifone, De Maria, Roncaioli et al.: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." In: The Journal of experimental medicine , Vol. 180, Issue 4, pp. 1547-52, 1994/01 (PubMed) Sergi, Kahl, Otto: "Contribution of apoptosis and apoptosis-related proteins to the malformation of the primitive intrahepatic biliary system in Meckel syndrome." In: The American journal of pathology , Vol. 156, Issue 5, pp. 1589-98, 2000/01 (PubMed) Zietz, Rumpler, Stuerzl et al.: "Inverse relation of Fas-ligand and tumor-infiltrating lymphocytes in angiosarcoma: indications of apoptotic tumor counterattack." In: The American journal of pathology , Vol. 159, Issue 3, pp. 963-70, 2001/01 (PubMed) |
Images: Fas (CD95) Human antibody
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Human peripheral blood lymphocytes stained with biotinylated DX2, followed by Sav-PE |
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