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应用范围
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Western Blotting (WB)
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应用备注
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 40kDa band observed in Human Heart lysates (calculated MW of 38.9kDa according to NP_001471.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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纯化
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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缓冲液
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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贮藏
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Aliquot and store at -20°C. Minimize freezing and thawing.
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限制
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For Research Use Only
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