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Product details for GFP antibody
GFP antibody
Overview
| Antigen: | Green Fluorescent Protein (GFP) |
| Antibody Type: | Polyclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC) (fro), Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB) |
| Host: | Goat |
| Quantity: | |
| Price: | 84,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN197865 |
| Availability: | Ships within 1 day |
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Additional Information: GFP antibody
| Antigen | Green Fluorescent Protein (GFP) |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length aminoacid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Antibody Type | Polyclonal |
| Format | Purified |
| Isotype | IgG »Matching secondary antibodies |
| Description | Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquoreavictoria, which emits green light (emission peak at a wavelenth of 509 nm) when excitedby blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein(GFP) has become an invaluable tool in cell biology research, since its intrinsicfluorescence can be visualized in living cells. GFP fluorescence is stable under fixationconditions and suitable for a variety of applications. GFP has been widely used as areporter for gene expression, enabling researchers to visualize and localize GFP-taggedproteins within living cells without the need for chemical staining. Other applications ofGFP include assessment of protein protein interactions through the yeast two hybridsystem and measurement of distance between proteins through fluorescence energytransfer (FRET) protocols. GFP technnology has considerably contributed to a greaterunderstanding of cellular physiology.YFP differs from GFP due to a mutation at T203Y,,, antibodies raised against full-lengthGFP should also detect YFP and other variants. Synonyms: Green Fluorescent Protein antibody |
| Host | Goat |
| Specificity | Anti-GFP assayed by ELISA for direct binding of antigen recognizes wild type,recombinant and enhanced forms of GFP. Minimal reaction with human, mouse, or rat serum proteins. |
Application Details: GFP antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC) (fro), Enzyme Immunoassay (EIA), Western Blotting (WB), Immunoblotting (IB) |
| Application Notes | Suggested working dilutions *ELISA 1:10,000-1:50,000,immunohistochemistry 1:500-1:3,000,Western Blot 1:1,000-1:5,000 * Investigator should determine optimal working dilutions. Optimal dilutions/concentrations should be determined by the end user. * Other applications have not been tested. |
| Format | Purified |
| Concentration | 1.15 mg/ml |
| Buffer | 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 0.1 mg sterile filtered antisera. |
| Storage | Store at 4 degrees C. Aliquot and store at -20 degrees C long-term. |
| Research Area | Tags/Labels |
| Restrictions | For Research Use only |
External Information: GFP antibody
| Google Scholar | Find more references for antigen Green Fluorescent Protein (GFP) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Green Fluorescent Protein (GFP) (Bioinformatic Harvester (EMBL)) |
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