Product details for  GST Tag antibody
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GST Tag antibody

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Antigen: GST Tag
Antibody Type: Polyclonal
Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)
Host: Rabbit
Quantity: 100ug
Price: 65,00 €   (Plus shipping costs and VAT)
Order number: ABIN195459
Availability: 2 days until shipment
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Antigen GST Tag
Immunogen Recombinant Glutathion-S-Transferase (GST) protein from Schistosoma japonicum
Antibody Type Polyclonal
Format Liquid
Description Determination of recombinant proteins containing the 23kD GST-tag of Schistosoma japonicum by Western Blotting, ELISA, Immunoprecipitation , Flow Cytometry and Immunohistochemistry
Host Rabbit

Application Details:  GST Tag antibody

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Application Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)
Purification This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with recombinant GST protein. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant GST-tag proteins by Western Blotting, Immunoprecipitation, ELISA, Flow Cytometry and Immunohistochemistry. Hereby it makes no difference if the GST-tag of the target protein is attached to the N- or the C-terminus.
Buffer TBS
Storage At least 18 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.
Research Area Bacteria, Tags/Labels
Restrictions For Research Use only

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Determination of a bacterially expressed GST protein with anti-GST-tag by Western Blot. A protein extract of an E.coli strain expressing the GST protein of Schistosoma japonicum (B) was resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The target protein was determined by antiGST-tag (1µg/ml) followed by Anti-Rabbit-HRP and ECL detection. The bands shown on the left represent a part of the Mark it West ECL protein standard of the MicroMol GmbH with individual bands of 10kD, 20kD, 30kD and 40kD respectively from bottom to the top.

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Google Scholar Find more references for antigen GST Tag on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen GST Tag (Bioinformatic Harvester (EMBL))