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Product details for Goat anti-Mouse IgA + IgG + IgM (H&L), Biotin

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Goat anti-Mouse IgA + IgG + IgM (H&L), Biotin

Overview

Antigen	IgA + IgG + IgM H&L
Immunogen	Mouse IgG, IgA and IgM whole molecules
Reactivity	Mouse (Murine)
Antibody Type	Polyclonal
Host	Goat
Specificity	This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. This ireagent is suitable for the detection of all mouse immunoglobulin subclasses, isotypes and chain combinations.

Application Notes	Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:16,000 of the reconstitution concentration is suggested for this product. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Conjugate	Biotin
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Buffer	This antibody is supplied as liquid IgG fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidoca
Storage	Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Research Area	Secondary Antibodies
Restrictions	Research only

Publications

Bayer, Wilchek:
"Protein biotinylation."
In: Methods in enzymology , Vol. 184, pp. 138-60, 1990/01 (PubMed)

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