Granzyme B (Human) antibody

Overview


	Antigen:	Granzyme B
	Antibody Type:	Monoclonal
	Application:	Flow Cytometry (FACS), Cell-ELISA, ELISA
	Reactivity:	Human
	Host:	Mouse
	Quantity:	100 ug
	Price:	250,00 € (Plus shipping costs and VAT)
	Order number:	ABIN108719
	Availability:	1 to 2 days until shipment

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Additional Information: Granzyme B (Human) antibody

Antigen	Granzyme B
Immunogen	GM4C1 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the granzyme B cDNA. It was purified by affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC cells trans
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Description	Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. GM4C1 reacts with human granzyme B (GrB), a 27 kDa serine protease which cleaves target-cell proteins at specific aspartate residues. In the target cell, granzyme B rapidly induces apoptosis by activating both caspase-mediated as well as caspase-independent pathways. Apart from the storage granules of cytotoxic cells granzymes can also be detected in biological fluids such as serum and plasma. Elevated levels of soluble granzymes have been found in the sera of patients with increased cytotoxic responses, caused by various viral infections, as well as in the synovial fluid of rheumatic arthritis patients. A high percentage of GrB positive CTL has also been found in the glands of patients suffering from Hodgkin`s disease and in CTL infiltrates in transplant rejection.
Clone	GM4C1
Host	Mouse
Specificity	GM4C1 recognises granzyme B transiently expressed on the cell surface of transfected BOSC cells (Fig. 1) as well as the native protein in peripheral blood mononuclear cells (Fig. 2). The antibody does not cross-react with the human granzymes A, K or M (Fi

Application Details: Granzyme B (Human) antibody

Application	Flow Cytometry (FACS), Cell-ELISA, ELISA
Application Notes	GM4C1 has been tested for the use in flow cytometry, ELISA and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal
Storage	Store the antibody at 4°C. Avoid repeated freezing and thawing.
Research Area	Apoptosis/Necrosis, Apoptosis/Necrosis, Inflammation
Restrictions	For research use only. Not for diagnostic or therapeutic use. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eye

Publications: Granzyme B (Human) antibody

Publications

Bade B, Lohrmann J, ten Brinke A et al.:
"Detection of soluble human granzyme K in vitro and in vivo"
In: Eur J Immunol , Vol. 10, pp. 2940-2948, 2007/01

Bade B, Boettcher HE, Lohrmann J et al.:
"Differential expression of the granzymes A, K, and M and perforin in human peripheral blood lymphocytes"
In: Int Immunol , Vol. 11, pp. 1419-1428

Images: Granzyme B (Human) antibody

Specificity testing of GM4C1. BOSC cells were transiently transfected with expression vectors for granzyme A, B, K and M as well as an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody (B, open curves)

Image for Granzyme B (Human) antibody (1)

Intracellular detection of granzyme B in human PBMC Mononuclear cells from peripheral blood (PBMC) were seperated by Ficoll-Hypaque, cultivated for 6h in the presence of phorbolester and ionomycin, fixed and permeabilised. Cells were incubated with hybr

Image for Granzyme B (Human) antibody (2)

External Information: Granzyme B (Human) antibody

Google Scholar	Find more references for clone GM4C1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Granzyme B (Bioinformatic Harvester (EMBL))