Product details for  H-2Db Mouse (Murine) antibody
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H-2Db Mouse (Murine) antibody

Overview

Antigen: H-2Db
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS)
Conjugate: Alexa Fluor 488
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN156568
Availability: Ships within 7 to 10 days
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Additional Information:  H-2Db Mouse (Murine) antibody

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Antigen H-2Db
Immunogen C57BL/10 mouse skin graft and splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2b, kappa Mouse (BALB/c) IgG2b, kappa »Matching secondary antibodies
Description The KH95 antibody reacts with the H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported. Distribution: Nucleated cells of the H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I
Clone KH95
Host Mouse

Application Details:  H-2Db Mouse (Murine) antibody

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Application Flow Cytometry (FACS)
Application Notes Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, and Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 488
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  H-2Db Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

York, Rock:
"Antigen processing and presentation by the class I major histocompatibility complex."
In: Annual review of immunology , Vol. 14, pp. 369-96, 1996/01 (PubMed)

Hasenkrug, Cory, Stimpfling:
"Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region."
In: Immunogenetics , Vol. 25, Issue 2, pp. 136-9, 1987/01 (PubMed)

Ponomarev, Shriver, Dittel:
"CD40 expression by microglial cells is required for their completion of a two-step activation process during central nervous system autoimmune inflammation."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 176, Issue 3, pp. 1402-10, 2006/01 (PubMed)

Shao, Peng, Liao et al.:
"A shared epitope of the interphotoreceptor retinoid-binding protein recognized by the CD4+ and CD8+ autoreactive T cells."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 175, Issue 3, pp. 1851-7, 2005/01 (PubMed)

External Information:  H-2Db Mouse (Murine) antibody

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Google Scholar Find more references for clone KH95 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen H-2Db (Bioinformatic Harvester (EMBL))