Product details for  H-2Kb Mouse (Murine) antibody
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H-2Kb Mouse (Murine) antibody

Overview

Image for H-2Kb Mouse (Murine) antibody
Antigen: H-2Kb
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 647
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN156876
Availability: Ships within 7 to 10 days
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Additional Information:  H-2Kb Mouse (Murine) antibody

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Antigen H-2Kb
Immunogen C57BL mouse splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (BALB/c) IgG2a, kappa »Matching secondary antibodies
Description The AF6-88.5 antibody reacts with H-2K MHC class I alloantigen expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v). Distribution: Nucleated cells of the H-2K haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I
Clone AF6-88-5
Host Mouse

Application Details:  H-2Kb Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 647
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  H-2Kb Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

York, Rock:
"Antigen processing and presentation by the class I major histocompatibility complex."
In: Annual review of immunology , Vol. 14, pp. 369-96, 1996/01 (PubMed)

Shao, Peng, Liao et al.:
"A shared epitope of the interphotoreceptor retinoid-binding protein recognized by the CD4+ and CD8+ autoreactive T cells."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 175, Issue 3, pp. 1851-7, 2005/01 (PubMed)

Wall, Lorber, Loken et al.:
"Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 131, Issue 3, pp. 1056-64, 1983/01 (PubMed)

Andersson, Freland, Johansson et al.:
"MHC class I mosaic mice reveal insights into control of Ly49C inhibitory receptor expression in NK cells."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 161, Issue 12, pp. 6475-9, 1999/01 (PubMed)

Loken, Stall:
"Flow cytometry as an analytical and preparative tool in immunology."
In: Journal of immunological methods , Vol. 50, Issue 3, pp. R85-112, 1982/01 (PubMed)

Images:  H-2Kb Mouse (Murine) antibody

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C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor(R) 647

Image for H-2Kb Mouse (Murine) antibody (1)

External Information:  H-2Kb Mouse (Murine) antibody

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Google Scholar Find more references for clone AF6-88-5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen H-2Kb (Bioinformatic Harvester (EMBL))