Product details for  H-2Kd Mouse (Murine) antibody
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H-2Kd Mouse (Murine) antibody

Overview

Image for H-2Kd Mouse (Murine) antibody
Antigen: H-2Kd
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 647
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 100 µg (Product variants)
Price: 226,00 €   (Plus shipping costs and VAT)
Order number: ABIN156889
Availability: Ships within 7 to 10 days
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Additional Information:  H-2Kd Mouse (Murine) antibody

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Antigen H-2Kd
Immunogen BALB/c Mouse cells
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (SJL) IgG2a, kappa »Matching secondary antibodies
Description The SF1-1.1 antibody reacts with the H-2K MHC class I alloantigens expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2, but does not cross-react with other haplotypes (b, j, p, q, s, v). Distribution: Nucleated cells of the H-2K haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I
Clone SF1-1-1
Host Mouse
Specificity Weakly cross-reacts with h-2 haplotype

Application Details:  H-2Kd Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 647
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  H-2Kd Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

York, Rock:
"Antigen processing and presentation by the class I major histocompatibility complex."
In: Annual review of immunology , Vol. 14, pp. 369-96, 1996/01 (PubMed)

Bashuda, Seino, Ra et al.:
"Lack of cognate help by CD4+ T cells and anergy of CD8+ T cells are the principal mechanisms for anti-leukocyte function-associated antigen-1/intercellular adhesion molecule-1-induced cardiac allograft tolerance."
In: Transplantation , Vol. 63, Issue 1, pp. 113-8, 1997/01 (PubMed)

Ma, Xu, An et al.:
"Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses."
In: Cancer research , Vol. 66, Issue 2, pp. 1169-76, 2006/01 (PubMed)

Norian, Allen:
"No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 173, Issue 2, pp. 835-44, 2004/01 (PubMed)

Noun, Reboul, Abastado et al.:
"Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 157, Issue 6, pp. 2455-61, 1996/01 (PubMed)

Images:  H-2Kd Mouse (Murine) antibody

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BALB/c mouse splenocytes stained with SF1-1.1 Alexa Fluor(R) 647

Image for H-2Kd Mouse (Murine) antibody (1)

External Information:  H-2Kd Mouse (Murine) antibody

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Google Scholar Find more references for clone SF1-1-1 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen H-2Kd (Bioinformatic Harvester (EMBL))