HBx antibody

Overview

Antigen:	Hepatitis B Virus Protein X (HBx)
Antibody Type:	Polyclonal
Application:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Host:	Rabbit
Quantity:	50 ug
Price:	330,00 € (Plus shipping costs and VAT)
Order number:	ABIN115505
Availability:	3 to 10 days until shipment

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Additional Information: HBx antibody

Antigen	Hepatitis B Virus Protein X (HBx)
Immunogen	Recombinant Hepatitis B Protein X from E. coli.
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	The recombinant Hepatitis B Protein X is 100% homologous with the natural Hepatitis B Protein X. Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.

Application Details: HBx antibody

Application	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes	Western Blot. ELISA: Titer is defined by indirect ELISA, it is: >1:100,000 for antibody concentration 1mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody was affinity purified on a column with immobilized recombinant Hepatitis B Protein X. Sterile filtered and lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. Add 0.05 ml of deionized water and let the lyophilized pellet
Storage	Store lyophilized antibody at 4°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C. Shelf life: one year from despatch.
Research Area	Virology
Restrictions	For Research Use only

Publications: HBx antibody

Publications

Zhang, Yang, Zhang et al.:
"Induction of hepatic enzymes and oxidative stress in Chinese rare minnow (Gobiocypris rarus) exposed to waterborne hexabromocyclododecane (HBCDD)."
In: Aquatic toxicology (Amsterdam, Netherlands) , Vol. 86, Issue 1, pp. 4-11, 2008/01 (PubMed)

External Information: HBx antibody

Google Scholar	Find more references for antigen Hepatitis B Virus Protein X (HBx) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Hepatitis B Virus Protein X (HBx) (Bioinformatic Harvester (EMBL))