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Product details for HIF-1a (Bovine) antibody
HIF-1a (Bovine) antibody
Overview
| Antigen: | Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) |
| Antibody Type: | Monoclonal |
| Application: | Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Monkey, Sheep (Ovine), Mouse (Murine), Rat, Rabbit, Pig (Porcine), Bovine, Ferret |
| Host: | Mouse |
| Quantity: | |
| Price: | 310,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN151032 |
| Availability: | Ships in 7 to 10 days |
Additional Information: HIF-1a (Bovine) antibody
| Antigen | Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) |
| Immunogen | Fusion protein containing amino acids 432-528 of human HIF-1alpha. Cellular Location: HIF-1 is a nuclear protein. |
| Reactivity | Human, Monkey, Sheep (Ovine), Mouse (Murine), Rat, Rabbit, Pig (Porcine), Bovine, Ferret |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2b »Matching secondary antibodies |
| Description | Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs asa posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimercomposed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantlytranslated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated atPro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventuallydegraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation preventsHIF degradation and increases transcriptional activity. Therefore, the concentration ofHIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under eithercondition. HIF hydroxylases provide insight into hypoxic cell responses, which may beused to help isolate therapeutic targets. Synonyms: Hypoxia-inducible factor 1 alpha antibody, HIF1 alpha antibody, ARNT interactingprotein antibody , Hif1a antibody, ARNT interacting protein antibody, HIF-1alphaantibody, Hypoxia inducible factor 1 alpha antibody, Hypoxia inducible factor 1 alphasubunit basic helix |
| Clone | H1alpha67 |
| Host | Mouse |
| Specificity | This antibody is specific for HIF-1 alpha. ABIN151032 reconizes human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferretHIF-1alpha. |
Application Details: HIF-1a (Bovine) antibody
| Application | Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | By Western blot, this antibody recognizes bands at 120 kDa representing HIF-1 alpha ininduced tissues and cells. Multiple bands may be seen at 120 kDa representingpost-translational modification of HIF-1alpha. This antibody has also been used toimmunoprecipitate human HIF-1 alpha. Suggested working dilution: *Western blot: 1/500 (ECL). Immunohistochemistry ** *The investigator should determine the optimal working dilution for a specificapplication. Nuclear extracts are recommended. ** Investigator should optimize their protocol for use of this antibody in IHC Positive Control:Nuclear extracts recommended. |
| Buffer | 0.25 ml of Protein G purified mouse ascites in PBS buffer and 0.05% sodium azide. PBS with 0.05% Sodium Azide. |
| Storage | Store at 4 deg C |
| Research Area | Transcription Factors |
| Restrictions | For Research Use only |
External Information: HIF-1a (Bovine) antibody
| Google Scholar | Find more references for clone H1alpha67 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) (Bioinformatic Harvester (EMBL)) |
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