产品详细信息：  HIF-1a (Bovine) antibody
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HIF-1a (Bovine) antibody

概述

抗原: Hypoxia-inducible Factor 1 alpha (HIF-1 alpha)
抗体类型: 单克隆抗体
应用范围: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
目标物种: Human, Monkey, Sheep (Ovine), Mouse (Murine), Rat, Rabbit, Pig (Porcine), Bovine, Ferret
抗体来源: Mouse
规格: 0.1 ml (3 mg/ml)
价格: 310,00 €   (加上运费和增值税)
货号: ABIN151053
发货时间: Ships in 7 to 10 days
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详细信息:  HIF-1a (Bovine) antibody

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抗原 Hypoxia-inducible Factor 1 alpha (HIF-1 alpha)
免疫原 Fusion protein containing amino acids 432-528 of human HIF-1alpha Cellular Location: HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular O2 concentration. Activates transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and other critical intracellular responses to hypoxia.
目标物种 Human, Monkey, Sheep (Ovine), Mouse (Murine), Rat, Rabbit, Pig (Porcine), Bovine, Ferret
抗体类型 单克隆抗体
状态 Purified
抗体同种型 IgG2b »相配的二抗
描述 Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Synonyms: Hypoxia-inducible factor 1 alpha antibody, HIF1 alpha antibody, ARNT interactingprotein antibody , Hif1a antibody, ARNT interacting protein antibody, HIF-1alphaantibody, Hypoxia inducible factor 1 alpha antibody, Hypoxia inducible factor 1 alphasubunit basic helix
克隆 H1alpha67
抗体来源 Mouse
特异性 This antibody is specific for HIF-1alpha. Recognizes human, sheep, mouse, rat, pig, bovine and ferret HIF-1 alpha.

使用细节:  HIF-1a (Bovine) antibody

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应用范围 Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
应用备注 By Western blot, recognizes bands at 120kDa representing HIF-1 alpha in inducedtissues and cells. Multiple bands may be seen at 120kDa representing post-translationalmodification. Can also be used for immunohistochemical detection of humanHIF-1alpha.Suggested working dilutions: * Western Blot (ECL) 1:500-1,000** Immunohistochemistry 1:500-1,000Immunoprecipitation - not determined*The investigator should determine the optimal working dilution for a specific application.** Nuclear Extracts.
浓度 3 mg/ml
缓冲液 0.1 ml of Protein G purified Cell Max culture supernatant in PBS and 0.05% sodium PBS with 0.05% sodium azide
贮藏 Store at 4 degrees C. Avoid repeated freeze-thaw cycles.
研究领域 Transcription Factors
限制 For Research Use only

外部连接:  HIF-1a (Bovine) antibody

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Google Scholar 克隆 H1alpha67
EMBL Harvester 在human, mouse 或 rat 基因组里搜索抗原Hypoxia-inducible Factor 1 alpha (HIF-1 alpha) (Bioinformatic Harvester (EMBL))