Product details for  HIS-tag antibody, HRP conjugated
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HIS-tag antibody, HRP conjugated

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Antigen: HIS-tag
Antibody Type: Polyclonal
Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)
Host: Rabbit
Quantity: 500ug (Product variants)
Price: 420,00 €   (Plus shipping costs and VAT)
Order number: ABIN195464
Availability: 2 days until shipment
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Additional Information:  HIS-tag antibody, HRP conjugated

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Antigen HIS-tag
Immunogen Cys – (Histidine)14
Antibody Type Polyclonal
Format Liquid
Description Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Flow Cytometry.
Host Rabbit

Application Details:  HIS-tag antibody, HRP conjugated

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Application Western Blotting (WB), ELISA, Flow Cytometry (FACS)
Purification This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Subsequently the antibody was conjugated to HRP by periodate coupling. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, ELISA and Flow Cytometry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.
Buffer PBS
Storage At least 12 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.
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Restrictions For Research Use only

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Determination of a bacterially expressed recombinant proteins with AntiHIS-tag-HRP by Western Blot. Protein extracts of E.coli strains expressing 3 individual recombinant proteins of 38kD, 50kD and 68kd (from right to the left) were resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The proteins were determined by AntiHis-tag-HRP (1µg/ml) in combination with colorimetric visualization. The numbers indiacted on the right represent the molecular weight of the protein standard used in the experiment.

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External Information:  HIS-tag antibody, HRP conjugated

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Google Scholar Find more references for antigen HIS-tag on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen HIS-tag (Bioinformatic Harvester (EMBL))