Product details for  HIS-tag antibody
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HIS-tag antibody

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Antigen: HIS-tag
Antibody Type: Polyclonal
Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)
Host: Rabbit
Quantity: 100ug (Product variants)
Price: 97,00 €   (Plus shipping costs and VAT)
Order number: ABIN195461
Availability: 2 days until shipment
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Additional Information:  HIS-tag antibody

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Antigen HIS-tag
Immunogen Cys – (Histidine)14
Antibody Type Polyclonal
Format Liquid
Description Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Immunoprecipitation , Flow Cytometry, Immunohistochemistry
Host Rabbit

Application Details:  HIS-tag antibody

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Application Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)
Purification This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, Immunoprecipitation, ELISA, Flow Cytometry and Immunohistochemistry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.
Buffer PBS
Storage At least 18 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.
Research Area Tags/Labels
Restrictions For Research Use only

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Determination of a bacterially expressed recombinant 40kD protein with antiHIS-tag by Western Blot. Protein extracts of a negative (A) and a positive bacterial strain (B) were resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The target protein was determined by antiHis-tag (1µg/ml) followed by Anti-Rabbit-HRP and ECL detection. The marks indicated on the left represent the prestained protein standard used in the experiment with individual bands of 23,1 kD, 31,3 kD, 53,6 kD and 100,4 kD respectively from bottom to the top.

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External Information:  HIS-tag antibody

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Google Scholar Find more references for antigen HIS-tag on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen HIS-tag (Bioinformatic Harvester (EMBL))