HSP27 antibody (pSer82) (Biotin)
Quick Overview for HSP27 antibody (pSer82) (Biotin) (ABIN126811)
Target
See all HSP27 (HSPB1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Binding Specificity
- pSer82
-
Specificity
- This antibody specifically interacts with hsp27 phosphorylated at serine 82 and does not crossreact with the non-phosphorylated hsp27 nor with unrelated serine-phosphorylated proteins.
-
Characteristics
- Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
-
Purification
- Size exclusion chromatography
-
Components
- incl. positive Control
-
Immunogen
- Synthetic phosphopeptide conjugated to KLH
-
Isotype
- IgG2a
-
-
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivity: Human, Rat WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 Reactivity: Human, Mouse, Rat WB, IF, FACS, IP, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivity: Human WB, IHC, ELISA, IF, FACS, IP Host: Rabbit Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 Reactivity: Human, Mouse, Rat, Sheep, Monkey, Chicken, Chimpanzee WB, IHC, IF, FACS Host: Mouse Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Biotin)HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 Biotin
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 HRP
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 390)HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 Atto 390
-
-
Application Notes
-
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from EGF-treated HepG2 cells. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.5 mg/mL
-
Buffer
- PBS/0.09 % Na-Azide/PEG and Sucrose
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C/-80 °C
-
Storage Comment
-
Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
-
-
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
-
Alternative Name
- HSPB1 / HSP27
-
Background
- The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
-
Gene ID
- 3315
-
UniProt
- P04792
-
Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Target
-
