BASKET
HELP
Product details for HSP27 pSer82 (Dog (Canine)) antibody
HSP27 pSer82 (Dog (Canine)) antibody
Overview
| Antigen: | Heat Shock Protein 27 (HSP27) pSer82 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Reactivity: | Dog (Canine), Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 330,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN126812 |
| Availability: | 3 to 10 days until shipment |
Additional Information: HSP27 pSer82 (Dog (Canine)) antibody
| Antigen | Heat Shock Protein 27 (HSP27) pSer82 |
| Immunogen | Synthetic phosphopeptide conjugated to KLH. |
| Reactivity | Dog (Canine), Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | Molecular weight of antigen is 27kDa. |
| Clone | 5B9 |
| Host | Mouse |
| Specificity | The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82. This antibody specifically interacts with hsp27 phosphorylated at serine 82 and does not crossreact with the non-phosphorylated hsp27 nor with unrelated serine-phosphorylated proteins. The antibody is suitable for Western blot and ELISA applications. Epitope: R Q L pS S G V. Reacts with human, mouse, rat and dog. |
Application Details: HSP27 pSer82 (Dog (Canine)) antibody
| Application | Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Application Notes | Western Blot: 0.5 ug/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.1 ug/ml (Potein-ELISA), capture ELISA: not determined. Positive control: HepG2 EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This purified antibody was lyophilized from 1 ml 2 x PBS with 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| Storage | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| Research Area | Metabolism, Heat Shock Proteins, Phospho-specific antibodies |
| Restrictions | For Research Use only |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
