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Product details for Haptoglobin (Human) antibody
Haptoglobin (Human) antibody
Overview
| Antigen: | Haptoglobin |
| Antibody Type: | Polyclonal |
| Application: | Immunoelectrophoresis (IEP), Immunofluorescence (IF), Radioimmunoassay (RIA), Rocket Immunoelectrophoresis (IEP) |
| Reactivity: | Human, Mouse (Murine), Rat, Goat, Dog (Canine), Rabbit |
| Host: | Sheep |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN148885 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Haptoglobin (Human) antibody
| Antigen | Haptoglobin |
| Immunogen | Human haptoglobin purified from serum. All Hp types (Hp1-1, Hp1-2 and Hp2-2) are present in the immunogen. |
| Reactivity | Human, Mouse (Murine), Rat, Goat, Dog (Canine), Rabbit |
| Antibody Type | Polyclonal |
| Isotype | IgG »Matching secondary antibodies |
| Description | Haptoglobin (Hp) is a plasma protein mostly synthesized in the liver. The haptoglobin monomer consists of two heavy, beta-chains (40 kDa) and two light, alpha-chains (alpha-1, 9 kDa, alpha-2, 16kDa) linked by disulphide bonds. Hp is a heterogeneous protein, the major variation being determined by two alleles at the alpha locus, namely alpha-1 and alpha-2. These associate to form three major Hp types, Hp1-1, Hp1-2 and Hp2-2. Hp1-1 exists as a monomer (alpha-1-beta) 2 (98kDa), Hp1-2 is polymeric (alpha-1-beta) m. (alpha-2-beta) n (about 200 kDa), as is Hp2-2 (alpha-2-beta) n (about 400 kDa). Plasma haptoglobin concentrations range from 1-3g/L. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine, which would lead to loss of iron. The Hp Hb complex is catabolized in the liver. Hp levels are decreased in hemolytic anemias and increased in a variety of inflammatory conditions. |
| Host | Sheep |
| Specificity | This product gives a single arc when tested by IEP and 2D-IEP against human serum. Identity is confirmed by double diffusion (Ouchterlony) vs human serum and a known anti-human haptoglobin. |
Application Details: Haptoglobin (Human) antibody
| Application | Immunoelectrophoresis (IEP), Immunofluorescence (IF), Radioimmunoassay (RIA), Rocket Immunoelectrophoresis (IEP) |
| Application Notes | RID and Rocket IEP: 2uL antiserum/cm?? gel vs 5uL neat-1/10 dilution human serumDouble diffusion: 10uL antiserum vs 5uL serumIEP: 100uL antiserum vs 5uL serumThe use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily beexcluded. |
| Concentration | Batch dependent |
| Purity | IgG fraction |
| Buffer | Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA |
| Storage | Store at 4 C where it will remain stable until the given expiry date. Slight precipitation can occur on storage which may be removed by centrifugation and should not affectperformance characteristics. |
| Research Area | Serum/Plasma Proteins |
| Restrictions | For research use only |
External Information: Haptoglobin (Human) antibody
| Google Scholar | Find more references for antigen Haptoglobin on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Haptoglobin (Bioinformatic Harvester (EMBL)) |
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