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Product details for  His 6x Epitope TAG Sequence: C-H-H-H-H-H-H antibody
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His 6x Epitope TAG Sequence: C-H-H-H-H-H-H antibody

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Antigen: His 6x Epitope TAG Sequence: C-H-H-H-H-H-H
Antibody Type: Polyclonal
Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Host: Rabbit
Quantity: 100ug (0.66 mg/ml (by UV absorbance a...)
Price: 268,33 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN100492
Availability: 7 days until shipment
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Additional Information:  His 6x Epitope TAG Sequence: C-H-H-H-H-H-H antibody

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Antigen His 6x Epitope TAG Sequence: C-H-H-H-H-H-H
Immunogen .
Antibody Type Polyclonal
Format Liquid (sterile filtered)
Host Rabbit

Application Details:  His 6x Epitope TAG Sequence: C-H-H-H-H-H-H antibody

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Application ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Concentration 0.66 mg/ml (by UV absorbance at 280 nm)
Purification This affinity purified antibody is directed against 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Storage Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Restrictions For Research Use only

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Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to separate the protein by SDSPAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye

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Google Scholar Find more references for antigen His 6x Epitope TAG Sequence: C-H-H-H-H-H-H on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen His 6x Epitope TAG Sequence: C-H-H-H-H-H-H (Bioinformatic Harvester (EMBL))