His-Tag antibody

Overview

Antigen:	His-Tag
Antibody Type:	Polyclonal
Application:	Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Host:	Rabbit
Quantity:	100 µg
Price:	361,63 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
Order number:	ABIN131281
Availability:	7 to 10 days until shipment

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Additional Information: His-Tag antibody

Antigen	His-Tag
Antibody Type	Polyclonal
Isotype	IgG, Ig »Matching secondary antibodies
Description	A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Host	Rabbit
Specificity	The affinity purified detects His-tagged recombinant proteins. It is expected that the His-Tag polyclonal antibody will react with proteins tagged with His from all species.

Application Details: His-Tag antibody

Application	Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes	The antibody can be used for Western blotting (1 µg/ml), immunoprecipitation (40 µg/ml), and immunocytochemistry (2 µg/ml). However, the optimal conditions should be determined individually.
Buffer	Supplied as 100 µg (0.5 mg/ml) affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% sodium azide.
Research Area	Tags/Labels
Restrictions	For Research Use Only

Publications: His-Tag antibody

Publications

Kroll, Abdel-Malek Abdel-Hafiz, Marcell et al.:
"A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection."
In: DNA and cell biology , Vol. 12, Issue 5, pp. 441-53, 1993/01 (PubMed)

External Information: His-Tag antibody

Google Scholar	Find more references for antigen His-Tag on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen His-Tag (Bioinformatic Harvester (EMBL))