Histone H1 azide free (Mam) antibody

概述

抗原:	Histone H1
抗体类型:	多克隆抗体
应用范围:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
目标物种:	Mammal
抗体来源:	Sheep
规格:	0.25 mg (1.21 mg/ml)
价格:	4.505,14 ¥ (加上运费和增值税, Invoice in EUR)
货号:	ABIN113038
发货时间:	3 to 10 days until shipment

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详细信息: Histone H1 azide free (Mam) antibody

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抗原	Histone H1
免疫原	Intact calf H-1 complexed to rRNA
目标物种	Mammal
抗体类型	多克隆抗体
状态	Azide Free
抗体来源	Sheep
特异性	This antibody specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. Cross-reactivity none found with other Histone protein H2a, H2b, H3 or H4.

使用细节: Histone H1 azide free (Mam) antibody

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应用范围	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
应用备注	Western blot: use at 0.1-1ug/ml with 2ug Histone H1. Immunoprecipitation. ELISA: The neat serum form of anti-histone-1 antibody can be titered to 1:64,000 but best used at 1:4,000 to 1:32,000. The antibody is 6.5 fold more active against histone-I than histone IIb. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
浓度	1.21 mg/ml
缓冲液	This antibody is supplied as Protein G purified (0.22um filtered), liquid Ig fraction in 0.01 M PBS pH 7.2 without preservatives..
贮藏	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
研究领域	DNA/RNA, Transcription Factors
限制	For Research Use only

参考文献: Histone H1 azide free (Mam) antibody

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参考文献

Stollar, Ward:
"Rabbit antibodies to histone fractions as specific reagents for preparative and comparative studies."
In: The Journal of biological chemistry , Vol. 245, Issue 6, pp. 1261-6, 1970/01 (PubMed)

Bustin, Stollar:
"Immunochemical specificity in lysine-rich histone subfractions."
In: The Journal of biological chemistry , Vol. 247, Issue 18, pp. 5716-21, 1972/01 (PubMed)

Neary, Mura, Stollar:
"Serological homologies between H1 degrees and H5 include the carboxyl-terminal domain."
In: The Journal of biological chemistry , Vol. 260, Issue 29, pp. 15850-5, 1986/01 (PubMed)