Histone H1 azide free (Mam) antibody

Übersicht

Antigen:	Histone H1
Antikörper-Typ:	Polyklonal
Applikation:	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Reaktivität:	Mammal
Wirt:	Sheep
Menge:	0.25 mg (1.21 mg/ml)
Preis:	490,00 € (Zzgl. Versandkosten und MWSt)
Bestellnummer:	ABIN113038
Verfügbarkeit:	3 to 10 days until shipment

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Produktbeschreibung: Histone H1 azide free (Mam) antibody

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Antigen	Histone H1
Immunogen	Intact calf H-1 complexed to rRNA
Reaktivität	Mammal
Antikörper-Typ	Polyklonal
Format	Azide Free
Wirt	Sheep
Spezifität	This antibody specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. Cross-reactivity none found with other Histone protein H2a, H2b, H3 or H4.

Anwendungen: Histone H1 azide free (Mam) antibody

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Applikation	Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Applikationshinweise	Western blot: use at 0.1-1ug/ml with 2ug Histone H1. Immunoprecipitation. ELISA: The neat serum form of anti-histone-1 antibody can be titered to 1:64,000 but best used at 1:4,000 to 1:32,000. The antibody is 6.5 fold more active against histone-I than histone IIb. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Konzentration	1.21 mg/ml
Buffer	This antibody is supplied as Protein G purified (0.22um filtered), liquid Ig fraction in 0.01 M PBS pH 7.2 without preservatives..
Lagerung	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Forschungsgebiet	DNA/RNA, Transcription Factors
Beschränkungen	For Research Use only

Publikationen: Histone H1 azide free (Mam) antibody

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Publikationen

Stollar, Ward:
"Rabbit antibodies to histone fractions as specific reagents for preparative and comparative studies."
In: The Journal of biological chemistry , Vol. 245, Issue 6, pp. 1261-6, 1970/01 (PubMed)

Bustin, Stollar:
"Immunochemical specificity in lysine-rich histone subfractions."
In: The Journal of biological chemistry , Vol. 247, Issue 18, pp. 5716-21, 1972/01 (PubMed)

Neary, Mura, Stollar:
"Serological homologies between H1 degrees and H5 include the carboxyl-terminal domain."
In: The Journal of biological chemistry , Vol. 260, Issue 29, pp. 15850-5, 1986/01 (PubMed)

Weitere Informationen: Histone H1 azide free (Mam) antibody

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