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Détail du produit pour Histone H1 azide free (Mam) antibody
Histone H1 azide free (Mam) antibody
Aperçu
| Antigène: | Histone H1 |
| Type anticorps: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivité: | Mammal |
| Hôte: | Sheep |
| Quantité: | |
| Prix: | 490,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN113038 |
| Disponibilité: | 3 to 10 days until shipment |
Description du produit: Histone H1 azide free (Mam) antibody
| Antigène | Histone H1 |
| Immunogène | Intact calf H-1 complexed to rRNA |
| Reactivité | Mammal |
| Type anticorps | Polyclonal |
| Format | Azide Free |
| Hôte | Sheep |
| Specificité | This antibody specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. Cross-reactivity none found with other Histone protein H2a, H2b, H3 or H4. |
Applications: Histone H1 azide free (Mam) antibody
| Application | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Indications d'application | Western blot: use at 0.1-1ug/ml with 2ug Histone H1. Immunoprecipitation. ELISA: The neat serum form of anti-histone-1 antibody can be titered to 1:64,000 but best used at 1:4,000 to 1:32,000. The antibody is 6.5 fold more active against histone-I than histone IIb. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1.21 mg/ml |
| Buffer | This antibody is supplied as Protein G purified (0.22um filtered), liquid Ig fraction in 0.01 M PBS pH 7.2 without preservatives.. |
| Stock | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Domaine de recherche | DNA/RNA, Transcription Factors |
| Restrictions | For Research Use only |
Références: Histone H1 azide free (Mam) antibody
| Références |
Stollar, Ward: "Rabbit antibodies to histone fractions as specific reagents for preparative and comparative studies." In: The Journal of biological chemistry , Vol. 245, Issue 6, pp. 1261-6, 1970/01 (PubMed) Bustin, Stollar: "Immunochemical specificity in lysine-rich histone subfractions." In: The Journal of biological chemistry , Vol. 247, Issue 18, pp. 5716-21, 1972/01 (PubMed) Neary, Mura, Stollar: "Serological homologies between H1 degrees and H5 include the carboxyl-terminal domain." In: The Journal of biological chemistry , Vol. 260, Issue 29, pp. 15850-5, 1986/01 (PubMed) |
Informations supplémentaires: Histone H1 azide free (Mam) antibody
| Google Scholar | Trouver des références supplémentaires pour antigène Histone H1 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène Histone H1 (Bioinformatic Harvester (EMBL)) |
