Product details for  Histone H1 azide free (Mam) antibody
Print Version Print Version       Download Datasheet Download Datasheet       Back to search results Back to search results       Add to basket Add to basket

Histone H1 azide free (Mam) antibody

Overview

Antigen: Histone H1
Antibody Type: Polyclonal
Application: Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Reactivity: Mammal
Host: Sheep
Quantity: 0.25 mg (1.21 mg/ml)
Price: 490,00 €   (Plus shipping costs and VAT)
Order number: ABIN113038
Availability: 3 to 10 days until shipment
Hints on ordering Hints on ordering
Add to basket

on this page

Additional Information
Application Details
Publications
Related Products
External Information

Customer Service

Technical Inquiry
Send product details to colleague
Download Datasheet

Additional Information:  Histone H1 azide free (Mam) antibody

back to top ^


Antigen Histone H1
Immunogen Intact calf H-1 complexed to rRNA
Reactivity Mammal
Antibody Type Polyclonal
Format Azide Free
Host Sheep
Specificity This antibody specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. Cross-reactivity none found with other Histone protein H2a, H2b, H3 or H4.

Application Details:  Histone H1 azide free (Mam) antibody

back to top ^


Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Application Notes Western blot: use at 0.1-1ug/ml with 2ug Histone H1. Immunoprecipitation. ELISA: The neat serum form of anti-histone-1 antibody can be titered to 1:64,000 but best used at 1:4,000 to 1:32,000. The antibody is 6.5 fold more active against histone-I than histone IIb. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1.21 mg/ml
Buffer This antibody is supplied as Protein G purified (0.22um filtered), liquid Ig fraction in 0.01 M PBS pH 7.2 without preservatives..
Storage Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area DNA/RNA, Transcription Factors
Restrictions For Research Use only

Publications:  Histone H1 azide free (Mam) antibody

back to top ^


Publications Stollar, Ward:
"Rabbit antibodies to histone fractions as specific reagents for preparative and comparative studies."
In: The Journal of biological chemistry , Vol. 245, Issue 6, pp. 1261-6, 1970/01 (PubMed)

Bustin, Stollar:
"Immunochemical specificity in lysine-rich histone subfractions."
In: The Journal of biological chemistry , Vol. 247, Issue 18, pp. 5716-21, 1972/01 (PubMed)

Neary, Mura, Stollar:
"Serological homologies between H1 degrees and H5 include the carboxyl-terminal domain."
In: The Journal of biological chemistry , Vol. 260, Issue 29, pp. 15850-5, 1986/01 (PubMed)

External Information:  Histone H1 azide free (Mam) antibody

back to top ^


Google Scholar Find more references for antigen Histone H1 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Histone H1 (Bioinformatic Harvester (EMBL))