Histone H1-ChIP Grade (Human) antibody

Overview

Antigen:	Histone H1-ChIP Grade
Antibody Type:	Monoclonal
Application:	ChIP assay, Immunocytochemistry (ICC), Western Blotting (WB)
Reactivity:	Human, Bovine
Host:	Mouse
Quantity:	50 µg (1 mg/ml)
Price:	335,80 $ (Plus shipping costs and VAT, Invoice in EUR)
Order number:	ABIN144214
Availability:	7 to 10 days until shipment

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Additional Information: Histone H1-ChIP Grade (Human) antibody

Antigen	Histone H1-ChIP Grade
Immunogen	Nuclei from myeloid leukemia biopsy cells.
Reactivity	Human, Bovine
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG2a »Matching secondary antibodies
Description	Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indic
Clone	0-T-60
Host	Mouse
Specificity	Doublet detected due to hyper-phosphorylation of histone H1 subpopulation.

Application Details: Histone H1-ChIP Grade (Human) antibody

Application	ChIP assay, Immunocytochemistry (ICC), Western Blotting (WB)
Application Notes	ChIP: Use 4-5ug for 10^6 cells. ICC: Use at a concentration of 5-20 mg/ml. WB: Use at a concentration of 1-4 ug/ml. Predicted molecular weight: 32-33 kDa. Detects histone H1 in lysates from an acid extract of HeLa cells. Lysate from acid extracted HeLa cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H1 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Concentration	1 mg/ml
Purity	Protein A purified
Buffer	PBS with 0.1% sodium azide, 40% glycerol
Storage	Antibody may be stored at 4 C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 C. Aliquots are stable for 24 months at -20 C. For maximum recovery of product, centrifuge the original vial af
Restrictions	For research use only

Publications: Histone H1-ChIP Grade (Human) antibody

Publications

Hebbes, Thorne, Crane-Robinson:
"A direct link between core histone acetylation and transcriptionally active chromatin."
In: The EMBO journal , Vol. 7, Issue 5, pp. 1395-402, 1988/01 (PubMed)

Monvall:
"[Students' action adopted at convention]"
In: Tidskrift för Sveriges sjuksköterskor , Vol. 42, Issue 22, pp. 23-4, 1976/01 (PubMed)

External Information: Histone H1-ChIP Grade (Human) antibody

Google Scholar	Find more references for clone 0-T-60 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Histone H1-ChIP Grade (Bioinformatic Harvester (EMBL))