Histone H1 antibody

概述


	抗原:	Histone H1
	抗体类型:	单克隆抗体
	应用范围:	Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
	抗体来源:	Mouse
	规格:	100 µg (1 mg/mL)
	价格:	1.985,94 ¥ (加上增值税, ¥91.94干冰费用和增值税, Invoice in EUR)
	货号:	ABIN130741
	发货时间:	7 to 10 days until shipment

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详细信息: Histone H1 antibody

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抗原	Histone H1
免疫原	This antibody was purified from hybridoma (clone C14093) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.
抗体类型	单克隆抗体
抗体同种型	IgG2b »相配的二抗
描述	The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. Histone H1, also known as linker histone, is bound to linker DNA region between nucleosomes, contributing to chromatin foldings in the nucleus. It is known that histone H1 is also post-translationally modified, such as phosphorylation or methylation and relation of these modifications with biological meanings are under extensive investigations. Histone H1 is essential for higher order structure of mitotic chromosomes that are condensed structure of chromatin during mitosis.
克隆	C14093
抗体来源	Mouse
特异性	This antibody reacts with Histone H1 (32kDa) on Western blotting. .

使用细节: Histone H1 antibody

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应用范围	Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
应用备注	Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 10 µg / 200 µL of cell extract from 5x10 6 cells. Immunocytochemistry: 1 µg / mL
浓度	1 mg/mL
缓冲液	100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.
研究领域	DNA/RNA, Transcription Factors
限制	For Research Use Only

参考文献: Histone H1 antibody

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参考文献

Higashi, Miyakawa, Uchiyama et al.:
"Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse."
In: Journal of biotechnology , Vol. 120, Issue 3, pp. 262-72, 2005/01 (PubMed)

Maresca, Freedman, Heald:
"Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts."
In: The Journal of cell biology , Vol. 169, Issue 6, pp. 859-69, 2005/01 (PubMed)

Uchiyama, Kobayashi, Takata et al.:
"Proteome analysis of human metaphase chromosomes."
In: The Journal of biological chemistry , Vol. 280, Issue 17, pp. 16994-7004, 2005/01 (PubMed)

Vettese-Dadey, Grant, Hebbes et al.:
"Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro."
In: The EMBO journal , Vol. 15, Issue 10, pp. 2508-18, 1996/01 (PubMed)

图像: Histone H1 antibody

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Western blot analysis of Histone H1 expression in HeLa cells (1), HL60 cells (2), A431 cells (3), WR19L cells (4) and PC12 cells (5) using ABIN130741.

图像 Histone H1 antibody (1)

Immunoprecipitation of Histone H1 from HeLa cells with mouse IgG2b (1) or ABIN130741 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN130741.

图像 Histone H1 antibody (2)