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Product details for Histone H3 (Mam) antibody
Histone H3 (Mam) antibody
Overview
| Antigen: | Histone H3 |
| Antibody Type: | Polyclonal |
| Application: | Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivity: | Mammal |
| Host: | Sheep |
| Quantity: | |
| Price: | 489,42 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN152166 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Histone H3 (Mam) antibody
| Antigen | Histone H3 |
| Immunogen | Histone 3, calf whole protein complexed to ribosomal RNA Cellular Location: Nuclear |
| Reactivity | Mammal |
| Antibody Type | Polyclonal |
| Format | Purified |
| Description | In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. Theyconsist of two major components in equal amounts, DNA and basic proteins calledhistones. Histones are believed to be regularly arranged in the deep grove of the DNAhelix. The recurring positive charges of the histones form electrostatic associations withthe negatively charged phosphate groups of DNA making the DNA more stable andflexible. This allows for the supercoiling of the chromatin fibers. |
| Host | Sheep |
| Specificity | Recognizes Histone H3: 100%No reaction with Histone-H-1 , Histone-H-2b orHistone-H-4Very low cross-reactivity with Histone-H-core Cross-reacts with a wide range of species. |
Application Details: Histone H3 (Mam) antibody
| Application | Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Enzyme-linked immunoshift assay: Use at an assay dependent concentration. Immunofluorscence: Use at an assay dependent dilution. Immunoprecipitation: 1:1000 - 4000. Western Blot: Use at an assay dependent concentration. Not tested in other applications.Optimal dilutions/concentrations should be determinedby the end user. Positive Control:Tonsil, colon carcinoma and any other human tissues. |
| Concentration | 2.5 mg/ml |
| Buffer | 0.125 mg Phosphate buffered saline |
| Storage | Store at -20 degrees C. |
| Research Area | DNA/RNA, Transcription Factors |
| Restrictions | For Research Use only |
External Information: Histone H3 (Mam) antibody
| Google Scholar | Find more references for antigen Histone H3 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 (Bioinformatic Harvester (EMBL)) |
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