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Product details for Histone H3 (acetyl-Lys9) (C. elegans) antibody
Histone H3 (acetyl-Lys9) (C. elegans) antibody
Overview
| Antigen: | Histone H3 (acetyl-Lys9) |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB), Immunofluorescence (IF) |
| Reactivity: | Human, Mouse (Murine), Rat, Drosophila, Caenorhabditis Elegens |
| Host: | Rabbit |
| Quantity: | |
| Price: | 462,23 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN151634 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Histone H3 (acetyl-Lys9) (C. elegans) antibody
| Antigen | Histone H3 (acetyl-Lys9) |
| Immunogen | Synthetic peptide corresponding to amino acids 7-20 of Histone H3 conjugated to KLH. |
| Reactivity | Human, Mouse (Murine), Rat, Drosophila, Caenorhabditis Elegens |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Description | Histone proteins H3, H4, H2A, and H2B function as building blocks to packageeukaryotic DNA into repeating nucleosome units that are folded in higherorderchromatin fibers.1,2 The nucleosome is composed of an octamer containing a H3/H4tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs ofDNA. The relatively unstructured and highly chargedN-terminal tail domains of histones, arecentral to the processes that modulate chromatin structure. A diverse and elaboratearray of post-translational modifications including acetylation, phosphorylation,methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domainsof histones.3,4 In addition, ATPdriven remodeling complexes, such as SWI/SNF, alterchromatin conformation.5,6 These modifications alter chromatin structure by influencinghistone-DNA and histone-histone interactions, and provide an exposed surface for thepotential interaction of the tail domain with other proteins involved in transcriptionregulation. Acetylation of lysine residues within these N-terminal domains by histoneacetyl-transferases (HATs), including Gcn5p, P/CAF, p300/CBP, and TAFII250, isassociated with transcriptional activation.2,7 This modification results in remodeling ofthe nucleosome structure into an open conformation more accessible to transcriptioncomplexes. Conversely, histone deacetylation by histone deacetylases (HDACs) isassociated with transcription repression reversing the chromatin remodeling process. Inmost species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23.3,8-11Acetylation at lysine 9 appears to have a dominant role in histone deposition andchromatin assembly in some organisms.8,12,13 Acetylation of specific lysine residues inH3 is also associated with processes apart from transcription. During DNA replication,new histones are rapidly synthesized and assembled onto replicated DNA. Histones H3and H4 are brought to replicating chromatin in a pre-acetylated state that turns into ade-acetylated state after replication is completed and the newly assembled chromatinmatures. Synonyms: H3, HisH3, His1H3, H3/a, H3/b, H3,c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HistoneH3 |
| Host | Rabbit |
| Specificity | Detects a band at approximately 17kDa. There is no inhibition with non-acetylatedHistone H3 peptide. This histone H3 sequence is identical in many species includingmouse, rat, bovine, chicken, frog, drosophila, and C. elegans. Cross-reacts with Human. Expected to cross-react with mouse, rat, bovine, chicken,frog, drosophila, and C. elegans. |
Application Details: Histone H3 (acetyl-Lys9) (C. elegans) antibody
| Application | Western Blotting (WB), Immunofluorescence (IF) |
| Application Notes | Suggested working dilutions *Western Blot, immunofluorescence * Investigator should determine optimal working dilutions. WB: Use at a dilution of 1/1,000. IF: Use at a dilution of 1/1,000. Not tested in any otherapplications. Optimal dilution should be determined by the end user. * Other applications have not been tested. Positive Control:Mouse fibroblast NIH3T3 cells treated with sodium butyrate. |
| Concentration | 2 mg/ml |
| Buffer | 0.01M PBS, pH 7.4 containing 15mM sodium azide 100 microliters |
| Storage | Store at 4 degress Celcius for up to one month. Aliquot and store at -20 degrees Celciuslong term. Avoid repeated freeze/thaw cycles. |
| Restrictions | For Research Use only |
External Information: Histone H3 (acetyl-Lys9) (C. elegans) antibody
| Google Scholar | Find more references for antigen Histone H3 (acetyl-Lys9) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 (acetyl-Lys9) (Bioinformatic Harvester (EMBL)) |
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