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Product details for Histone H3 azide free (Mam) antibody
Histone H3 azide free (Mam) antibody
Overview
| Antigen: | Histone H3 |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Mammal |
| Host: | Sheep |
| Quantity: | |
| Price: | 666,15 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN113040 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Histone H3 azide free (Mam) antibody
| Antigen | Histone H3 |
| Immunogen | Intact calf H-3 complexed with rRNA |
| Reactivity | Mammal |
| Antibody Type | Polyclonal |
| Format | Azide Free |
| Host | Sheep |
| Specificity | Histone-III by Western blot and immunoprecipitation: MW 15.3kD. In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and the basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histone-III is moderately rich in arginine and contains cysteine. |
Application Details: Histone H3 azide free (Mam) antibody
| Application | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Suitable for use in ELISA, Western blot and immunoprecipitation. Has been screened by immunoprecipitation (2.5-10.0 ug/ml) Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1.6mg/ml (OD280nm, E0.1% = 1.36) |
| Buffer | This antibody is supplied as liquid, Protein G affinity purified Ig Fraction. in PBS pH 7.2 without preservatives. |
| Storage | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | DNA/RNA, Transcription Factors |
| Restrictions | For Research Use only |
Publications: Histone H3 azide free (Mam) antibody
| Publications |
Lejnine, Durfee, Murnane et al.: "Crosslinking of proteins to DNA in human nuclei using a 60 femtosecond 266 nm laser." In: Nucleic acids research , Vol. 27, Issue 18, pp. 3676-84, 1999/01 (PubMed) |
External Information: Histone H3 azide free (Mam) antibody
| Google Scholar | Find more references for antigen Histone H3 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 (Bioinformatic Harvester (EMBL)) |
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