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Product details for Histone H3 pSer10 (Human) antibody
Histone H3 pSer10 (Human) antibody
Overview
| Antigen: | Histone H3 pSer10 |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 557,39 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN152953 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Histone H3 pSer10 (Human) antibody
| Antigen | Histone H3 pSer10 |
| Immunogen | Synthetic peptide: ARTKQTARKSpTGGKAPRK conjugated to KLH,corresponding to amino acids 1-18 of Human Histone H3. Cellular Location: Nuclear |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | This antibody is a useful tool to study molecular mechanisms associated with the G2 toM transition and chromatin condensation and for the screening of in vivo inhibitors ofkinase(s) or phosphatase(s) involved in H3 phosphorylation ordephosphorylation.Mitogenic stimulation, oncogen transformation, orinduction ofoncogenic ras expression is accompanied with increases in Ser-10 phosphorylation ofthe N-terminal domain H3 (ref 5).Indeed, it has been shown that phosphorylated H3 isassociated with c-fos and c-myc genes in stimulated cells (ref 6).H3 phosphorylationmay contribute to proto-oncogen induction by modulating chromatin structure andreleasing blocks in elongation.Site-specific phosphorylation of histone H3 at serine 10(Ser10) appears to occur exclusively during mitosis in mammalian cells. H3dephosphorylation occurs quite rapidly after mitosis and Ser10 remainsunphosphorylated throughout the remainder of interphase (See Ajiro et al. and Wolffe). |
| Clone | H3-P |
| Host | Mouse |
| Specificity | The antibody reacts with human histone H3 (See Juan et al. 1998 and 1999),phosphorylated near its N-terminus (at Ser10) and does not detect thenon-phosphorylated epitope (See Juan et al.). Cross-reacts with Human.Not yet tested in other species. |
Application Details: Histone H3 pSer10 (Human) antibody
| Application | Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Dot: Use at an assay dependant concentration. FACS: Use at a concentration of 50 -300 ug/ml. ICC: Use at an assay dependant concentration. (See Juan et al. 1998 and1999)IF: Use at an assay dependant dilution. (See Sauve et al.)I-ELISA: Use at anassay dependant dilution. (See Juan et al. 1998 and Sauve et al.)WB: Use at an assaydependant concentration. (See Sauve et al.)Not tested in other applications.Optimaldilutions/concentrations should be determined by the end user.We advise you tocentrifuge this product vial before use. |
| Concentration | 2 mg/ml |
| Buffer | 100 micrograms Liquid 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/mlBSA |
| Storage | Store at 4°C. Aliquot and store at -20°C long-term. |
| Restrictions | For Research Use only |
External Information: Histone H3 pSer10 (Human) antibody
| Google Scholar | Find more references for clone H3-P on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 pSer10 (Bioinformatic Harvester (EMBL)) |
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