Histone H3 (phospho S10) (Human) antibody

Overview

Antigen:	Histone H3 (phospho S10)
Antibody Type:	Monoclonal
Application:	Dot Blot (Dot), Flow Cytometry (FACS), indirect ELISA (I-ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Reactivity:	Human
Host:	Mouse
Quantity:	100 µg (2.2 mg/ml)
Price:	368,42 $ (Plus shipping costs and VAT, Invoice in EUR)
Order number:	ABIN145104
Availability:	7 to 10 days until shipment

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Additional Information: Histone H3 (phospho S10) (Human) antibody

Antigen	Histone H3 (phospho S10)
Immunogen	Synthetic peptide: ARTKQTARKSpTGGKAPRK conjugated to KLH, corresponding to amino acids 1-18 of Human Histone H3.
Reactivity	Human
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG2a »Matching secondary antibodies
Description	This antibody is a useful tool to study molecular mechanisms associated with the G2 to M transition and chromatin condensation, and for the screening of in vivo inhibitors of kinase(s) or phosphatase(s) involved in H3 phosphorylation or dephosphorylation. Mitogenic stimulation, oncogene transformation, or induction of oncogenic ras expression is accompanied with increases in Ser-10 phosphorylation of the N-terminal domain H3 (ref 5). Indeed, it has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells (ref 6). H3 phosphorylation may contribute to proto-oncogene induction by modulating chromatin structure and releasing blocks in elongation. Site-specific phosphorylation of histone H3 at serine 10 (Ser10) appears to occur exclusively during mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly after mitosis and Ser10 remains unphosphorylated throughout the remainder of interphase (See Ajiro et al. and Wolffe).
Clone	H3-P
Host	Mouse
Specificity	The antibody reacts with human histone H3 (See Juan et al. 1998 and 1999), phosphorylated near its N-terminus (at Ser10) and does not detect the non-phosphorylated epitope (See Juan et al.).

Application Details: Histone H3 (phospho S10) (Human) antibody

Application	Dot Blot (Dot), Flow Cytometry (FACS), indirect ELISA (I-ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Application Notes	Dot: Use at an assay dependant concentration. FACS: Use at a concentration of 100-300 ug/ml. ICC: Use at an assay dependant concentration. (See Juan et al. 1998 and 1999)IF: Use at an assay dependant dilution. (See Sauve et al. )I-ELISA: Use at an assay dependant dilution. (See Juan et al. 1998 and Sauve et al. )WB: Use at an assay dependant concentration. (See Sauve et al. )Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. We advise you to centrifuge this product vial before use.
Concentration	2.2 mg/ml
Purity	IgG fraction
Buffer	0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Storage	Store at 4 C. Aliquot and store at -20 C long-term.
Restrictions	For research use only

Publications: Histone H3 (phospho S10) (Human) antibody

Publications

Wei, Yu, Bowen et al.:
"Phosphorylation of histone H3 is required for proper chromosome condensation and segregation."
In: Cell , Vol. 97, Issue 1, pp. 99-109, 1999/01 (PubMed)

Chadee, Hendzel, Tylipski et al.:
"Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts."
In: The Journal of biological chemistry , Vol. 274, Issue 35, pp. 24914-20, 1999/01 (PubMed)

Gurley, Walters, Tobey:
"Sequential phsophorylation of histone subfractions in the Chinese hamster cell cycle."
In: The Journal of biological chemistry , Vol. 250, Issue 10, pp. 3936-44, 1975/01 (PubMed)

Shibata, Inagaki, Ajiro:
"Mitosis-specific histone H3 phosphorylation in vitro in nucleosome structures."
In: European journal of biochemistry / FEBS , Vol. 192, Issue 1, pp. 87-93, 1990/01 (PubMed)

Ajiro, Nishimoto:
"Specific site of histone H3 phosphorylation related to the maintenance of premature chromosome condensation. Evidence for catalytically induced interchange of the subunits."
In: The Journal of biological chemistry , Vol. 260, Issue 29, pp. 15379-81, 1986/01 (PubMed)

Sauve, Anderson, Ray et al.:
"Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic chromosome condensation."
In: The Journal of cell biology , Vol. 145, Issue 2, pp. 225-35, 1999/01 (PubMed)

Juan, Traganos, James et al.:
"Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis."
In: Cytometry , Vol. 32, Issue 2, pp. 71-7, 1998/01 (PubMed)

Juan, Traganos, Darzynkiewicz:
"Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation."
In: Experimental cell research , Vol. 246, Issue 1, pp. 212-20, 1999/01 (PubMed)

External Information: Histone H3 (phospho S10) (Human) antibody

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EMBL Harvester	Search human, mouse or rat genome for antigen Histone H3 (phospho S10) (Bioinformatic Harvester (EMBL))