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Product details for Histone H3 antibody
Histone H3 antibody
Overview
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| Antigen: | Histone H3 |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Mouse (Murine), Human |
| Host: | Rabbit |
| Quantity: | |
| Price: | 353,47 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN160705 |
| Availability: | Ships within 7 to 10 days |
Additional Information: Histone H3 antibody
| Antigen | Histone H3 |
| Immunogen | Recombinant (partial), N-terminal |
| Reactivity | Mouse (Murine), Human |
| Antibody Type | Polyclonal |
| Format | Purified |
| Isotype | Rabbit IgG »Matching secondary antibodies |
| Description | Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. The Poly6019 antibody has been shown to be useful for Western blotting of human and mouse histone H3. Distribution: Nuclear Function: Component of octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). Creates nucleosome structure of chromosomal fiber in eukaryotes, dynamic gene regulation Modifications: Phosphorylation, Acetylation, Ubiquitination, Ribosylation, Methylation Regulation: Cell cycle-dependent transcriptional regulation except for variant H3.3, cell cycle-independent Structure: 17 kD |
| Clone | Poly6019 |
| Host | Rabbit |
| Specificity | Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, CARM1. |
Application Details: Histone H3 antibody
| Application | Western Blotting (WB) |
| Application Notes | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by antigen-affinity chromatography. |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
| Storage | Upon receipt, store frozen at -20° C. |
| Research Area | DNA/RNA, Transcription Factors |
| Restrictions | For Research Use only |
Publications: Histone H3 antibody
| Publications |
Bauer, Daujat, Nielsen et al.: "Methylation at arginine 17 of histone H3 is linked to gene activation." In: EMBO reports , Vol. 3, Issue 1, pp. 39-44, 2002/01 (PubMed) Frank, Doenecke, Albig: "Differential expression of human replacement and cell cycle dependent H3 histone genes." In: Gene , Vol. 312, pp. 135-43, 2003/01 (PubMed) Strahl, Allis: "The language of covalent histone modifications." In: Nature , Vol. 403, Issue 6765, pp. 41-5, 2000/01 (PubMed) Wu, Bonner: "Separation of basal histone synthesis from S-phase histone synthesis in dividing cells." In: Cell , Vol. 27, Issue 2 Pt 1, pp. 321-30, 1982/01 (PubMed) |
Images: Histone H3 antibody
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Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-histone H3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. |
External Information: Histone H3 antibody
| Google Scholar | Find more references for clone Poly6019 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 (Bioinformatic Harvester (EMBL)) |
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