Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes prior to antibody staining. Cells were incubated with a 1:50 dilution of Histone H4 antibody (Poly6020) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor(R) 488 (top panel). After washing twice in 0.1% Triton X-100 in PBS, cells were mounted and examined on a fluorescent scope. DAPI stained cells are shown as a control (bottom panel). 100 X magnification. Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Histone H4 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Histone H4 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Histone H4 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.