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Product details for Hsp27 (Human) antibody
Hsp27 (Human) antibody
Overview
| Antigen: | Hsp27 |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA |
| Reactivity: | Human, Dog (Canine), Pig (Porcine), Mink |
| Host: | Rabbit |
| Quantity: | |
| Price: | 416,01 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR) |
| Order number: | ABIN130809 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Hsp27 (Human) antibody
| Antigen | Hsp27 |
| Reactivity | Human, Dog (Canine), Pig (Porcine), Mink |
| Antibody Type | Polyclonal |
| Isotype | IgG, Ig »Matching secondary antibodies |
| Description | CD40 is a type I membrane protein found on the Human Hsp27, mouse Hsp25 and aß-crystallin are part of a diverse family of small heat shock proteins that are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanine, glycine or aspartate, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3. |
| Host | Rabbit |
| Specificity | Recognizes HSP27 in human and monkey. Weakly recognizes HSP27 in dog and pig samples. The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, dog (weakly) and pig (weakly) origins. |
Application Details: Hsp27 (Human) antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA |
| Application Notes | The antibody can be used for Western blotting (1-2 µg/ml), immunoprecipitation (5-10 µg/ml), immunocytochemistry (10-15 µg/ml), and ELISA (1 µg/ml). However, the optimal conditions should be determined individually. |
| Buffer | Supplied as 100 µg (200 µg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide. |
| Research Area | Heat Shock Proteins |
| Restrictions | For Research Use Only |
External Information: Hsp27 (Human) antibody
| Google Scholar | Find more references for antigen Hsp27 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Hsp27 (Bioinformatic Harvester (EMBL)) |
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