Hsp27 (Human) antibody

Overview

Antigen:	Hsp27
Antibody Type:	Polyclonal
Application:	Western Blotting (WB)
Reactivity:	Human, Mouse (Murine), Rat, Hamster
Host:	Rabbit
Quantity:	100 µL (Product variants)
Price:	300,45 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR)
Order number:	ABIN132004
Availability:	7 to 10 days until shipment

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Additional Information: Hsp27 (Human) antibody

Antigen	Hsp27
Immunogen	This antibody was affinity purified from rabbit polyclonal antibody raised against HSP 27 purified from human skeletal muscle.
Reactivity	Human, Mouse (Murine), Rat, Hamster
Antibody Type	Polyclonal
Format	Liquid
Description	The human HSP27 is detected in various normal tissues, and it is present at high levels in muscle tissues. It has been found that HSP27 effectively prevent heat-induced aggregation of other proteins. These observations suggest that HSP27 also function as molecular chaperons.
Host	Rabbit
Specificity	This antibody detects 27~28 kDa of human HSP 27 on western blotting.

Application Details: Hsp27 (Human) antibody

Application	Western Blotting (WB)
Application Notes	SDS-PAGE and Western Blotting 1) Boil the sample for 3~5 minutes and centrifuge at 12,000 x g for a minute. Other methods may be employable. 2) Resolve 10 µ l of sample by SDS-polyacrylamide gel electrophoresis (See the manufacture's manual for electrophoresis condition). 3) Transfer to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system. (Transfer Buffer - 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 4) The transferred proteins can be visualized by staining the membrane for 1 minute with Ponceau (SIGMA, Cat# P-7170). Rinse the membrane with PBS. 5) Non-specific binding sites are blocked by immersing the membrane in BlockAce™ for 1 hour at room temperature (20~25 o C) or for overnight at 4 o C. 6) Incubate in primary antibody diluted with PBS containing 10% BlockAce™ as suggested in the Applications for 1 hour at room temperature (20~25 o C). (The concentration of the antibody to be used will be dependent on condition.) 7) Wash the membrane 3 times with PBS containing 0.05% Tween20 for 5~10 minutes each. 8) Incubate the membrane with secondary antibody (1:8,000 diluted POD conjugated anti rabbit IgG (H+L) in PBS containing 10 % Blockace) for 1 hour at room temperature (20~25 o C). 9) Wash the membrane 3 times with PBS containing 0.05% Tween20 for 5~10 minutes each. 10) Wipe excess buffer from the membrane, then incubate it with appropriate chemiluminescence reagent for 1 minute. Remove extra reagent from the membrane by dabbing with paper towel, and seal it in plastic wrap. 11) Expose to a X-ray film in a dark room for 30 seconds. Develop the film as usual. The conditions for exposure and development may vary. Western blotting: 1:1,000 dilution Details concerning the following research applications are provided.
Buffer	PBS containing 50% glycerol and 1 mg/ml of BSA. No preservative is contained.
Research Area	Heat Shock Proteins
Restrictions	For Research Use Only

Publications: Hsp27 (Human) antibody

Publications

Inaguma, Goto, Shinohara et al.:
"Physiological and pathological changes in levels of the two small stress proteins, HSP27 and alpha B crystallin, in rat hindlimb muscles."
In: Journal of biochemistry , Vol. 114, Issue 3, pp. 378-84, 1994/01 (PubMed)

Product variants

Hsp27 (Human) antibody (Quantity: 100 µg)

External Information: Hsp27 (Human) antibody

Google Scholar	Find more references for antigen Hsp27 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Hsp27 (Bioinformatic Harvester (EMBL))