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Product details for Hsp70 (Human) antibody
Hsp70 (Human) antibody
Overview
| Antigen: | Hsp70 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
| Reactivity: | Human, Mouse (Murine), Rat, Monkey, Bovine, Rabbit, Hamster, Pig (Porcine), Dog (Canine), Sheep (Ovine), Chicken, Mink |
| Host: | Mouse |
| Quantity: | |
| Price: | 416,01 $ (Plus shipping costs , $13.59 dry ice fee and VAT, Invoice in EUR) |
| Order number: | ABIN130812 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Hsp70 (Human) antibody
| Antigen | Hsp70 |
| Reactivity | Human, Mouse (Murine), Rat, Monkey, Bovine, Rabbit, Hamster, Pig (Porcine), Dog (Canine), Sheep (Ovine), Chicken, Mink |
| Antibody Type | Monoclonal |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus, b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus, c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum, and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. |
| Clone | S5 |
| Host | Mouse |
| Specificity | The antibody recognizes Hsp70 in human, mouse, rat, monkey, bovine, rabbit, hamster, guinea pig, dog, sheep, chicken, and pig cell lysates. The antibody recognizes a 70 kDa protein, corresponding to the apparent molecular weight of the inducible Hsp70 on SDS-PAGE immunoblots. This antibody has no reactivity with the constitutive Hsc70 (Hsp73). |
Application Details: Hsp70 (Human) antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) |
| Application Notes | The antibody can be used for Western blot analysis (1 µg/ml), immunoprecipitation (5-10 µg/ml), immuno- histochemistry (5-10 µg/ml), and FACS analysis. However, the optimal conditions should be determined individually. |
| Buffer | Supplied as 100 µg (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol. |
| Research Area | Heat Shock Proteins |
| Restrictions | For Research Use Only |
Publications: Hsp70 (Human) antibody
| Publications |
Welch, Suhan: "Cellular and biochemical events in mammalian cells during and after recovery from physiological stress." In: The Journal of cell biology , Vol. 103, Issue 5, pp. 2035-52, 1987/01 (PubMed) |
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