I Kappa B alpha (Human) antibody

Overview


	Antigen:	I Kappa B alpha (IKBa)
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	280,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123497
	Availability:	Ships in 3 to 10 days

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Additional Information: I Kappa B alpha (Human) antibody

Antigen	I Kappa B alpha (IKBa)
Immunogen	A synthetic peptide corresponding to amino acids 34-48 of human IkBa was used as the immunogen. It will cross-react with mouse and rat IkBa, based on BLAST.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Purified
Host	Rabbit
Specificity	NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkBa. This antibody will react with human IkBa. Predicted React : Mouse, Rat.

Application Details: I Kappa B alpha (Human) antibody

Application	Western Blotting (WB)
Application Notes	Western blot (1 - 3 ug/ml). In Jurkat, a 40 kDa band is observed. Optimal dilutions of this antibody are dependent on application and should be determined by the user. Western blot analysis of IkBa in 20 ug of whole Jurkat cell lysate using ABIN123497 at 2 ug/ml.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as Protein G purified IgG PBS containing 0.2% gelatin and 0.05% sodium azide.
Storage	Store the antibody at 4°C for one month or in aliquots at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions	Research only

Publications: I Kappa B alpha (Human) antibody

Publications

Verma, Stevenson:
"IkappaB kinase: beginning, not the end."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 94, Issue 22, pp. 11758-60, 1997/01 (PubMed)

DiDonato, Hayakawa, Rothwarf et al.:
"A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB."
In: Nature , Vol. 388, Issue 6642, pp. 548-54, 1997/01 (PubMed)

Mercurio, Zhu, Murray et al.:
"IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation."
In: Science (New York, N.Y.) , Vol. 278, Issue 5339, pp. 860-6, 1997/01 (PubMed)

Yamaoka, Courtois, Bessia et al.:
"Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation."
In: Cell , Vol. 93, Issue 7, pp. 1231-40, 1998/01 (PubMed)

Images: I Kappa B alpha (Human) antibody

Western blot analysis of IkBa in 20 ug of whole Jurkat cell lysate using ABIN123497 at 2 ug/ml.

Image for I Kappa B alpha (Human) antibody (1)

External Information: I Kappa B alpha (Human) antibody

Google Scholar	Find more references for antigen I Kappa B alpha (IKBa) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen I Kappa B alpha (IKBa) (Bioinformatic Harvester (EMBL))