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Product details for I Kappa B alpha (IKBa) (Human) antibody
I Kappa B alpha (IKBa) (Human) antibody
Overview
| Antigen: | I Kappa B alpha (IKBa) |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 340,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN116524 |
| Availability: | Ships in 3 to 10 days |
Additional Information: I Kappa B alpha (IKBa) (Human) antibody
| Antigen | I Kappa B alpha (IKBa) |
| Immunogen | IkBa peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Polyclonal |
| Format | Serum |
| Host | Rabbit |
| Specificity | Recognition is at the highly conserved C-terminal region. The antibody detects hypo- and hyper-phosphorylated forms of IkB-alpha. Reacts with human, mouse and rat. Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting. |
Application Details: I Kappa B alpha (IKBa) (Human) antibody
| Application | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Western blotting: 1:1,000 - shows a 36kD band. Immunoprecipitation. ELISA. Dot Blot. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 90 mg/ml (by Refractometry) |
| Buffer | This antibody is supplied as liquid sterile filtered antiserum with 0.01% Sodium Azide as preservative. This product was prepared from monospecific antiserum by delipidation and defibrination. |
| Storage | Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Restrictions | Research only |
External Information: I Kappa B alpha (IKBa) (Human) antibody
| Google Scholar | Find more references for antigen I Kappa B alpha (IKBa) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen I Kappa B alpha (IKBa) (Bioinformatic Harvester (EMBL)) |
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