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产品详细信息： I-A/I-E Mouse (Murine) antibody
I-A/I-E Mouse (Murine) antibody
概述
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| 抗原: | I-A/I-E |
| 抗体类型: | 单克隆抗体 |
| 应用范围: | Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC) |
| 偶联物: | PE |
| 目标物种: | Mouse (Murine) |
| 抗体来源: | Rat |
| 规格: | |
| 价格: | 3.074,66 ¥ (加上运费和增值税, Invoice in EUR) |
| 货号: | ABIN156277 |
| 发货时间: | Ships within 7 to 10 days |
详细信息: I-A/I-E Mouse (Murine) antibody
| 抗原 | I-A/I-E |
| 免疫原 | Activated C57BL/6 mouse spleen cells |
| 目标物种 | Mouse (Murine) |
| 抗体类型 | 单克隆抗体 |
| 抗体同种型 | Rat IgG2b, kappa »相配的二抗 |
| 描述 | The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II |
| 克隆 | M5-114-15-2 |
| 抗体来源 | Rat |
| 特异性 | Reacts with a shared determinant on i-a |
使用细节: I-A/I-E Mouse (Murine) antibody
| 应用范围 | Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC) |
| 应用备注 | Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| 偶联物 | PE |
| 纯化 | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody. |
| 缓冲液 | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| 贮藏 | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| 限制 | For Research Use only |
参考文献: I-A/I-E Mouse (Murine) antibody
| 参考文献 |
Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed) Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed) Kim, Noh, Kim et al.: "Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination." In: Experimental & molecular medicine , Vol. 36, Issue 5, pp. 428-43, 2004/01 (PubMed) Luckashenak, Ryszkiewicz, Ramsey et al.: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 177, Issue 8, pp. 5177-85, 2006/01 (PubMed) Nelson, Hosier, Brady et al.: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 151, Issue 5, pp. 2453-61, 1993/01 (PubMed) Shi, Kaliyaperumal, Lu et al.: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." In: The Journal of experimental medicine , Vol. 187, Issue 3, pp. 367-78, 1998/01 (PubMed) Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." In: Cell , Vol. 72, Issue 4, pp. 635-48, 1993/01 (PubMed) Yamashita, Nagata, Tada et al.: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." In: International immunology , Vol. 5, Issue 9, pp. 1139-50, 1994/01 (PubMed) Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 127, Issue 6, pp. 2488-95, 1982/01 (PubMed) |
相似抗体
外部连接: I-A/I-E Mouse (Murine) antibody
| Google Scholar | 克隆 M5-114-15-2 |
| EMBL Harvester | 在human, mouse 或 rat 基因组里搜索抗原I-A/I-E (Bioinformatic Harvester (EMBL)) |
