产品详细信息：  I-A/I-E Mouse (Murine) antibody
打印版 打印版       电子版下载 电子版下载       回搜索结果 回搜索结果       放入购物车 放入购物车

I-A/I-E Mouse (Murine) antibody

概述

抗原: I-A/I-E
抗体类型: 单克隆抗体
应用范围: Flow Cytometry (FACS), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Antibody (BLOCK)
偶联物: LEAF
目标物种: Mouse (Murine)
抗体来源: Rat
规格: 50 µg (相似抗体)
价格: 916,48 ¥   (加上运费和增值税, Invoice in EUR)
货号: ABIN156278
发货时间: Ships within 7 to 10 days
订购提示 订购提示
放入购物车

本页更多信息

详细信息
使用细节
参考文献
相关产品
外部连接

客户服务

发送技术问题
转发产品信息给同事
电子版下载

详细信息:  I-A/I-E Mouse (Murine) antibody

回页首 ^


抗原 I-A/I-E
免疫原 Activated C57BL/6 mouse spleen cells
目标物种 Mouse (Murine)
抗体类型 单克隆抗体
状态 Sterile Low-Endotoxin, Azide-Free formulation (LEAF)
抗体同种型 Rat IgG2b, kappa »相配的二抗
描述 The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Synonyms: MHC class II Structure: MHC class II
克隆 M5-114-15-2
抗体来源 Rat
特异性 Reacts with a shared determinant on i-a

使用细节:  I-A/I-E Mouse (Murine) antibody

回页首 ^


应用范围 Flow Cytometry (FACS), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Antibody (BLOCK)
应用备注 Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
偶联物 LEAF
纯化 The antibody was purified by affinity chromatography.
缓冲液 Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
贮藏 The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
限制 For Research Use only

参考文献:  I-A/I-E Mouse (Murine) antibody

回页首 ^


参考文献 Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Kim, Noh, Kim et al.:
"Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination."
In: Experimental & molecular medicine , Vol. 36, Issue 5, pp. 428-43, 2004/01 (PubMed)

Luckashenak, Ryszkiewicz, Ramsey et al.:
"The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 177, Issue 8, pp. 5177-85, 2006/01 (PubMed)

Nelson, Hosier, Brady et al.:
"Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 151, Issue 5, pp. 2453-61, 1993/01 (PubMed)

Shi, Kaliyaperumal, Lu et al.:
"Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain."
In: The Journal of experimental medicine , Vol. 187, Issue 3, pp. 367-78, 1998/01 (PubMed)

Viville, Neefjes, Lotteau et al.:
"Mice lacking the MHC class II-associated invariant chain."
In: Cell , Vol. 72, Issue 4, pp. 635-48, 1993/01 (PubMed)

Yamashita, Nagata, Tada et al.:
"CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection."
In: International immunology , Vol. 5, Issue 9, pp. 1139-50, 1994/01 (PubMed)

Bhattacharya, Dorf, Springer:
"A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 127, Issue 6, pp. 2488-95, 1982/01 (PubMed)

外部连接:  I-A/I-E Mouse (Murine) antibody

回页首 ^


Google Scholar 克隆 M5-114-15-2
EMBL Harvester 在human, mouse 或 rat 基因组里搜索抗原I-A/I-E (Bioinformatic Harvester (EMBL))