Product details for  I-A/I-E Mouse (Murine) antibody
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I-A/I-E Mouse (Murine) antibody

Overview

Antigen: I-A/I-E
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 488
Reactivity: Mouse (Murine)
Host: Rat
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN156284
Availability: Ships within 7 to 10 days
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Additional Information:  I-A/I-E Mouse (Murine) antibody

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Antigen I-A/I-E
Immunogen Activated C57BL/6 mouse spleen cells
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Rat IgG2b, kappa »Matching secondary antibodies
Description The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II
Clone M5-114-15-2
Host Rat
Specificity Reacts with a shared determinant on i-a

Application Details:  I-A/I-E Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 488
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  I-A/I-E Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Kim, Noh, Kim et al.:
"Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination."
In: Experimental & molecular medicine , Vol. 36, Issue 5, pp. 428-43, 2004/01 (PubMed)

Luckashenak, Ryszkiewicz, Ramsey et al.:
"The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 177, Issue 8, pp. 5177-85, 2006/01 (PubMed)

Nelson, Hosier, Brady et al.:
"Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 151, Issue 5, pp. 2453-61, 1993/01 (PubMed)

Shi, Kaliyaperumal, Lu et al.:
"Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain."
In: The Journal of experimental medicine , Vol. 187, Issue 3, pp. 367-78, 1998/01 (PubMed)

Viville, Neefjes, Lotteau et al.:
"Mice lacking the MHC class II-associated invariant chain."
In: Cell , Vol. 72, Issue 4, pp. 635-48, 1993/01 (PubMed)

Yamashita, Nagata, Tada et al.:
"CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection."
In: International immunology , Vol. 5, Issue 9, pp. 1139-50, 1994/01 (PubMed)

Bhattacharya, Dorf, Springer:
"A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 127, Issue 6, pp. 2488-95, 1982/01 (PubMed)

External Information:  I-A/I-E Mouse (Murine) antibody

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Google Scholar Find more references for clone M5-114-15-2 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I-A/I-E (Bioinformatic Harvester (EMBL))