Product details for  I-Ab (Abetab) Mouse (Murine) antibody
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I-Ab (Abetab) Mouse (Murine) antibody

Overview

Antigen: I-Ab (Abetab)
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 488
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 100 µg (Product variants)
Price: 226,00 €   (Plus shipping costs and VAT)
Order number: ABIN156641
Availability: Ships within 7 to 10 days
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Additional Information:  I-Ab (Abetab) Mouse (Murine) antibody

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Antigen I-Ab (Abetab)
Immunogen C3H.SW splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (C3H) IgG2a, kappa »Matching secondary antibodies
Description The 25-9-17 antibody reacts with the beta chain of the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2 haplotypes. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: ß chain of I-A MHC class II
Clone 25-09-2017
Host Mouse
Specificity Cross-reacts with alloantigens of h-2 haplotypes

Application Details:  I-Ab (Abetab) Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156644). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 488
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  I-Ab (Abetab) Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Cohn, Glimcher, Waldmann et al.:
"Identification of functional regions on the I-Ab molecule by site-directed mutagenesis."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 83, Issue 3, pp. 747-51, 1986/01 (PubMed)

Khanna, Morelli, Zhong et al.:
"Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 164, Issue 3, pp. 1346-54, 2000/01 (PubMed)

Meuter, Schaerli, Roos et al.:
"Murine CXCL14 is dispensable for dendritic cell function and localization within peripheral tissues."
In: Molecular and cellular biology , Vol. 27, Issue 3, pp. 983-92, 2007/01 (PubMed)

Nikolic, Gardner, Scadden et al.:
"Normal development in porcine thymus grafts and specific tolerance of human T cells to porcine donor MHC."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 162, Issue 6, pp. 3402-7, 1999/01 (PubMed)

Ozato, Sachs:
"Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 126, Issue 1, pp. 317-21, 1981/01 (PubMed)

Wall, Lorber, Loken et al.:
"Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 131, Issue 3, pp. 1056-64, 1983/01 (PubMed)

External Information:  I-Ab (Abetab) Mouse (Murine) antibody

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Google Scholar Find more references for clone 25-09-2017 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I-Ab (Abetab) (Bioinformatic Harvester (EMBL))