Product details for  I-Ab Mouse (Murine) antibody
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I-Ab Mouse (Murine) antibody

Overview

Antigen: I-Ab
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 647
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN156726
Availability: Ships within 7 to 10 days
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Additional Information:  I-Ab Mouse (Murine) antibody

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Antigen I-Ab
Immunogen C57BL/10 mouse splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (BALB/c) IgG2a, kappa »Matching secondary antibodies
Description The KH74 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-A, I-A, I-A, and I-A haplotypes. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II
Clone KH74
Host Mouse

Application Details:  I-Ab Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemical staining of acetone-fixed frozen tissue sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 647
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  I-Ab Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Hasenkrug, Cory, Stimpfling:
"Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region."
In: Immunogenetics , Vol. 25, Issue 2, pp. 136-9, 1987/01 (PubMed)

Pappo, Torrey, Castriotta et al.:
"Helicobacter pylori infection in immunized mice lacking major histocompatibility complex class I and class II functions."
In: Infection and immunity , Vol. 67, Issue 1, pp. 337-41, 1999/01 (PubMed)

External Information:  I-Ab Mouse (Murine) antibody

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Google Scholar Find more references for clone KH74 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I-Ab (Bioinformatic Harvester (EMBL))