Product details for  I-Ab Mouse (Murine) antibody
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I-Ab Mouse (Murine) antibody

Overview

Antigen: I-Ab
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 488
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 100 µg (Product variants)
Price: 226,00 €   (Plus shipping costs and VAT)
Order number: ABIN156862
Availability: Ships within 7 to 10 days
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Additional Information:  I-Ab Mouse (Murine) antibody

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Antigen I-Ab
Immunogen C57BL/10J splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (BALB/c) IgG2a, kappa »Matching secondary antibodies
Description The AF6-120.1 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 and H-2 haplotypes, this antibody does not cross-react with other haplotypes (d, f, q, r, s). Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II
Clone AF6-120-1
Host Mouse

Application Details:  I-Ab Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 488
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  I-Ab Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Buono, Come, Stavrakis et al.:
"Influence of interferon-gamma on the extent and phenotype of diet-induced atherosclerosis in the LDLR-deficient mouse."
In: Arteriosclerosis, thrombosis, and vascular biology , Vol. 23, Issue 3, pp. 454-60, 2003/01 (PubMed)

Cohn, Glimcher, Waldmann et al.:
"Identification of functional regions on the I-Ab molecule by site-directed mutagenesis."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 83, Issue 3, pp. 747-51, 1986/01 (PubMed)

Nakagawa, Brissette, Lira et al.:
"Impaired invariant chain degradation and antigen presentation and diminished collagen-induced arthritis in cathepsin S null mice."
In: Immunity , Vol. 10, Issue 2, pp. 207-17, 1999/01 (PubMed)

Wall, Lorber, Loken et al.:
"Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 131, Issue 3, pp. 1056-64, 1983/01 (PubMed)

Wang, Castellaneta, De Creus et al.:
"Heart, but not skin, allografts from donors lacking Flt3 ligand exhibit markedly prolonged survival time."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 172, Issue 10, pp. 5924-30, 2004/01 (PubMed)

External Information:  I-Ab Mouse (Murine) antibody

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Google Scholar Find more references for clone AF6-120-1 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I-Ab (Bioinformatic Harvester (EMBL))