Product details for  I-Ak (Abetak) Mouse (Murine) antibody
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I-Ak (Abetak) Mouse (Murine) antibody

Overview

Antigen: I-Ak (Abetak)
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Conjugate: Alexa Fluor 647
Reactivity: Mouse (Murine)
Host: Mouse
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN156490
Availability: Ships within 7 to 10 days
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Additional Information:  I-Ak (Abetak) Mouse (Murine) antibody

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Antigen I-Ak (Abetak)
Immunogen C3H mouse splenocytes
Reactivity Mouse (Murine)
Antibody Type Monoclonal
Isotype Mouse IgG2a, kappa Mouse (CWB) IgG2a, kappa »Matching secondary antibodies
Description The 10-3.6 antibody reacts with the beta chain of the I-A MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-A antigens, does not react with other haplotypes (e.g., b, d, p, q). Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: ß chain of MHC class II
Clone 10-03-2006
Host Mouse

Application Details:  I-Ak (Abetak) Mouse (Murine) antibody

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Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
Application Notes Additional reported applications (for the relevant formats) include immunoprecipitation, protection against autoimmune IDDM, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 647
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Restrictions For Research Use only

Publications:  I-Ak (Abetak) Mouse (Murine) antibody

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Publications Pamer, Cresswell:
"Mechanisms of MHC class I--restricted antigen processing."
In: Annual review of immunology , Vol. 16, pp. 323-58, 1998/01 (PubMed)

Watts:
"Capture and processing of exogenous antigens for presentation on MHC molecules."
In: Annual review of immunology , Vol. 15, pp. 821-50, 1997/01 (PubMed)

Alisauskas, Ponzio:
"In vivo growth inhibition of Ia+ lymphomas in SJL mice treated with I-As-reactive monoclonal antibody."
In: Immunopharmacology , Vol. 12, Issue 1, pp. 1-9, 1986/01 (PubMed)

Kappler, Skidmore, White et al.:
"Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition."
In: The Journal of experimental medicine , Vol. 153, Issue 5, pp. 1198-214, 1981/01 (PubMed)

Kupinski, Plunkett, Freed:
"Assignment of antigenic determinants to separated I-A kappa chains."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 130, Issue 5, pp. 2277-81, 1983/01 (PubMed)

External Information:  I-Ak (Abetak) Mouse (Murine) antibody

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EMBL Harvester Search human, mouse or rat genome for antigen I-Ak (Abetak) (Bioinformatic Harvester (EMBL))