Product details for  IKBa (Human) antibody
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IKBa (Human) antibody

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Antigen: I Kappa B alpha (IKBa)
Antibody Type: Monoclonal
Application: Immunoprecipitation (IP), Western Blotting (WB)
Reactivity: Human, Mouse (Murine)
Host: Mouse
Quantity: 0.1 mg (0.5 mg/ml)
Price: 280,00 €   (Plus shipping costs and VAT)
Order number: ABIN121247
Availability: Ships in 3 to 10 days
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Additional Information:  IKBa (Human) antibody

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Antigen I Kappa B alpha (IKBa)
Immunogen A recombinant protein corresponding to amino acid residues 32-391 of human IkBa was used as immunogen.
Reactivity Human, Mouse (Murine)
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone 6A920
Host Mouse
Specificity NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa. Reacts with mouse and human.

Application Details:  IKBa (Human) antibody

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Application Immunoprecipitation (IP), Western Blotting (WB)
Application Notes Immunoprecipitation: 1 µg/ml. Western blot analysis (1.0 - 2.0 µg/ml, positive control: Daudi, Jurkat, or NIH 3T3). A 40 kDa band is observed. The antibody reacts with human and mouse IkBa. It may react with other species, but this has not been tested. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. Western blot analysis of IkBa using ABIN121247 at 1 µg/mL against 30 µg of Jurkat total cell lysate. Western blot analysis of IkBa using ABIN121247 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.
Concentration 0.5 mg/ml
Buffer This antibody is supplied as liquid IgG fraction, purified on Protein G, in PBS containing 0.02% sodium azide as preservative.
Storage Store the antibody solution at 4°C. DO NOT FREEZE.
Restrictions Research only

Publications:  IKBa (Human) antibody

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Publications Sanjo, Kawai, Akira:
"DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis."
In: The Journal of biological chemistry , Vol. 273, Issue 44, pp. 29066-71, 1998/01 (PubMed)

Moriguchi, Urushiyama, Hisamoto et al.:
"WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1."
In: The Journal of biological chemistry , Vol. 280, Issue 52, pp. 42685-93, 2005/01 (PubMed)

Verma, Stevenson:
"IkappaB kinase: beginning, not the end."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 94, Issue 22, pp. 11758-60, 1997/01 (PubMed)

DiDonato, Hayakawa, Rothwarf et al.:
"A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB."
In: Nature , Vol. 388, Issue 6642, pp. 548-54, 1997/01 (PubMed)

Mercurio, Zhu, Murray et al.:
"IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation."
In: Science (New York, N.Y.) , Vol. 278, Issue 5339, pp. 860-6, 1997/01 (PubMed)

Yamaoka, Courtois, Bessia et al.:
"Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation."
In: Cell , Vol. 93, Issue 7, pp. 1231-40, 1998/01 (PubMed)

Images:  IKBa (Human) antibody

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Western blot analysis of IkBa using ABIN121247 at 1 ug/mL against 30 ug of Jurkat total cell lysate.

Image for IKBa (Human) antibody (1)

Western blot analysis of IkBa using ABIN121247 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate.

Image for IKBa (Human) antibody (2)

External Information:  IKBa (Human) antibody

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Google Scholar Find more references for clone 6A920 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I Kappa B alpha (IKBa) (Bioinformatic Harvester (EMBL))