Product details for  IKKg (Human) antibody
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IKKg (Human) antibody

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Antigen: I Kappa K gamma (IKKg)
Antibody Type: Monoclonal
Application: Western Blotting (WB)
Reactivity: Human, Mouse (Murine)
Host: Mouse
Quantity: 0.1 mg (0.5 mg/ml)
Price: 280,00 €   (Plus shipping costs and VAT)
Order number: ABIN121252
Availability: Ships in 3 to 10 days
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Additional Information:  IKKg (Human) antibody

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Antigen I Kappa K gamma (IKKg)
Immunogen This antibody was raised against a His-tagged full-length human IKK3/IKKg protein.
Reactivity Human, Mouse (Murine)
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone 72C627
Host Mouse
Specificity NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-7). IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator) (7). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg (8,9). This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb (8). Reacts with Mouse and Human.

Application Details:  IKKg (Human) antibody

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Application Western Blotting (WB)
Application Notes Western blot analysis (2.0 µg/ml, positive control: Jurkat or NIH 3T3). A 48 kDa band is observed. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Buffer This antibody is supplied as liquid IgG fraction, purified on Protein G, in PBS containing 0.02% sodium azide as preservative.
Storage Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions Research only

Publications:  IKKg (Human) antibody

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Publications Verma, Stevenson, Schwarz et al.:
"Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation."
In: Genes & development , Vol. 9, Issue 22, pp. 2723-35, 1995/01 (PubMed)

DiDonato, Hayakawa, Rothwarf et al.:
"A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB."
In: Nature , Vol. 388, Issue 6642, pp. 548-54, 1997/01 (PubMed)

Jin, Giordano, Kibler et al.:
"Role of adapter function in oncoprotein-mediated activation of NF-kappaB. Human T-cell leukemia virus type I Tax interacts directly with IkappaB kinase gamma."
In: The Journal of biological chemistry , Vol. 274, Issue 25, pp. 17402-5, 1999/01 (PubMed)

Mercurio, Zhu, Murray et al.:
"IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation."
In: Science (New York, N.Y.) , Vol. 278, Issue 5339, pp. 860-6, 1997/01 (PubMed)

Regnier, Song, Gao et al.:
"Identification and characterization of an IkappaB kinase."
In: Cell , Vol. 90, Issue 2, pp. 373-83, 1997/01 (PubMed)

Yamaoka, Courtois, Bessia et al.:
"Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation."
In: Cell , Vol. 93, Issue 7, pp. 1231-40, 1998/01 (PubMed)

Harhaj, Sun:
"IKKgamma serves as a docking subunit of the IkappaB kinase (IKK) and mediates interaction of IKK with the human T-cell leukemia virus Tax protein."
In: The Journal of biological chemistry , Vol. 274, Issue 33, pp. 22911-4, 1999/01 (PubMed)

Verma, Stevenson:
"IkappaB kinase: beginning, not the end."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 94, Issue 22, pp. 11758-60, 1997/01 (PubMed)

Images:  IKKg (Human) antibody

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Western blot analysis of 30 ug of total cell lysate from Jurkat (lane 1) and NIH-3T3 (lane 2) cells with ABIN121252 at 2 ug/ml dilution.

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External Information:  IKKg (Human) antibody

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Google Scholar Find more references for clone 72C627 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen I Kappa K gamma (IKKg) (Bioinformatic Harvester (EMBL))