Product details for  IL-1 beta (Human) antibody
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IL-1 beta (Human) antibody

Overview

Antigen: Interleukin-1 beta (IL-1 beta)
Antibody Type: Monoclonal
Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (FUNC)
Reactivity: Human
Host: Mouse
Quantity: 0.1 mg (1.0 mg/ml)
Price: 230,00 €   (Plus shipping costs and VAT)
Order number: ABIN121260
Availability: Ships in 3 to 10 days
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Additional Information:  IL-1 beta (Human) antibody

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Antigen Interleukin-1 beta (IL-1 beta)
Immunogen A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen.
Reactivity Human
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone 2D8
Host Mouse
Specificity Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8. Reacts with Mouse, Human.

Application Details:  IL-1 beta (Human) antibody

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Application Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (FUNC)
Application Notes Immunohistochemistry on frozen sections: please see reference (3, 4) for details. Clone 2D8 was employed to study the expression of IL-1b on cryopreserved sections of rectal mucosal tissue from patients with shigellosis (4), and on frozen sections of tissue from children with tonsillitis (4). Immunofluorescence/Immunocytochemistry: 1-10 ug/ml (2). Immunocytochemistry (ICC): This antibody was shown to be useful for intracellular staining of cultured human peripheral blood derived and endotoxin stimulated monocytes by indirect immunofluorescence at a concentraton of 1-10 ug/ml (2). Neutralization: please see reference (5) for details. This antibody is also useful for the neutralization of the biological activity of IL-1b as demonstrated by the ability to inhibit the IL-1b induced and not the IL-1a induced IL-2 production in EL4 cells (5). ELISA: 10 ug/ml. This antibody can be used as the capture reagent in a two-site sandwich ELISA for measuring human IL-1b levels in conjunction with biotinylated anti-human IL-1b polyclonal antibody as the detecting antibody, and recombinant human IL-1b as the standard. Optimal concentration for the capture monoclonal (clone 2D8) was 10 ug/ml, this ELISA measured natural human IL-1b in gingival tissue from patients with periodontitis with a detection limit of 8 pg/ml (6). An ELISA using this antibody was also useful in the determination of IL-1b levels in the sinovial fluid from patients with rheumatoid arthritis (6). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Buffer This antibody is supplied as liquid IgG fraction, purified on Protein G, in PBS containing 0.02% sodium azide as preservative.
Storage Store the antibody at 4°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Cytokines, Receptors, Inflammation
Restrictions Research only

Publications:  IL-1 beta (Human) antibody

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Publications Andersson, Bjoerk, Dinarello et al.:
"Lipopolysaccharide induces human interleukin-1 receptor antagonist and interleukin-1 production in the same cell."
In: European journal of immunology , Vol. 22, Issue 10, pp. 2617-23, 1992/01 (PubMed)

Raqib, Lindberg, Bjoerk et al.:
"Down-regulation of gamma interferon, tumor necrosis factor type I, interleukin 1 (IL-1) type I, IL-3, IL-4, and transforming growth factor beta type I receptors at the local site during the acute phase of Shigella infection."
In: Infection and immunity , Vol. 63, Issue 8, pp. 3079-87, 1995/01 (PubMed)

Towbin, Schmitz, van Oostrum et al.:
"Monoclonal antibody based enzyme-linked and chemiluminescent assays for the human interleukin-1 receptor antagonist. Application to measure hIL-1ra levels in monocyte cultures and synovial fluids."
In: Journal of immunological methods , Vol. 170, Issue 1, pp. 125-35, 1994/01 (PubMed)

External Information:  IL-1 beta (Human) antibody

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Google Scholar Find more references for clone 2D8 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Interleukin-1 beta (IL-1 beta) (Bioinformatic Harvester (EMBL))