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Product details for IL-12/IL-23 p40 Human antibody
IL-12/IL-23 p40 Human antibody
Overview
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| Antigen: | IL-12/IL-23 p40 |
| Antibody Type: | Monoclonal |
| Application: | ELISA (detection), ELISPOT (detection) |
| Conjugate: | Biotin |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 361,63 $ (Plus shipping costs and VAT, Invoice in EUR) |
| Order number: | ABIN160581 |
| Availability: | Ships within 7 to 10 days |
Additional Information: IL-12/IL-23 p40 Human antibody
| Antigen | IL-12/IL-23 p40 |
| Immunogen | COS-expressed p40 subunit of human IL-12 |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Isotype | Mouse IgG1, kappa »Matching secondary antibodies |
| Description | IL-12 p70 (p40:p35) induces IFN-gamma, TNF-a production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-gamma by human memory T cells, but not naive T cells. In the mouse, IL-23 has been reported to induce the proinflammatory cytokine IL-17 in memory T cells. Both IL-12 and IL-23 can induce the production of IL-12 in splenic dendritic cells in mice, however, only more mature dendritic cells (CD8) are able to respond to IL-23. Dendritic cells, monocytes/macrophages, B cells, T cells The C8.6 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C8.6 antibody has been reported to strongly inhibit different biological activities of IL-12, (e.g., IFN-gamma induction, mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity). The C8.6 antibody can neutralize the bioactivity of natural or recombinant IL-12. Synonyms: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF) Regulation: Downregulated by IL-10, homodimeric p40 antagonistic to functional p70 heterodimer, p40 monomer has no function, p40 subunit in common with IL-23 Structure: Cytokine, monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40) |
| Clone | C8-6 |
| Host | Mouse |
Application Details: IL-12/IL-23 p40 Human antibody
| Application | ELISA (detection), ELISPOT (detection) |
| Application Notes | ELISA or ELISPOT Detection: The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p40 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the C8.3 antibody (ABIN160175/ABIN160177) as the capture antibody. The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 7B12 antibody (ABIN160624/ABIN160626) as the capture antibody. Biotin-F(ab') (ABIN160584) is recommended for ELISA analysis of human serum and plasma samples, to prevent non-specific binding of interfering substances such as rheumatoid factor and heterophilic antibodies. Each lot of this antibody is quality control tested by ELISA assay. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of human IL-12 bioactivity (ABIN160583). Additional reported applications (for the relevant format) include: immunoprecipitation, Western blotting. No. to) are specially developed and recommended. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | Biotin |
| Purification | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C. Do not freeze. |
| Research Area | Cytokines, Immunology, Virology, Inflammation, Cancer |
| Restrictions | For Research Use only |
Publications: IL-12/IL-23 p40 Human antibody
| Publications |
Aggarwal, Ghilardi, Xie et al.: "Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17." In: The Journal of biological chemistry , Vol. 278, Issue 3, pp. 1910-4, 2003/01 (PubMed) Belladonna, Renauld, Bianchi et al.: "IL-23 and IL-12 have overlapping, but distinct, effects on murine dendritic cells." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 168, Issue 11, pp. 5448-54, 2002/01 (PubMed) Lankford, Frucht: "A unique role for IL-23 in promoting cellular immunity." In: Journal of leukocyte biology , Vol. 73, Issue 1, pp. 49-56, 2003/01 (PubMed) Oppmann, Lesley, Blom et al.: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." In: Immunity , Vol. 13, Issue 5, pp. 715-25, 2001/01 (PubMed) Parham, Chirica, Timans et al.: "A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R." In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 168, Issue 11, pp. 5699-708, 2002/01 (PubMed) Quesniaux: "Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview." In: Research in immunology , Vol. 143, Issue 4, pp. 385-400, 1992/01 (PubMed) Trinchieri, Wysocka, DAndrea et al.: "Natural killer cell stimulatory factor (NKSF) or interleukin-12 is a key regulator of immune response and inflammation." In: Progress in growth factor research , Vol. 4, Issue 4, pp. 355-68, 1993/01 (PubMed) Trinchieri: "Interleukin-12 and its role in the generation of TH1 cells." In: Immunology today , Vol. 14, Issue 7, pp. 335-8, 1993/01 (PubMed) |
Product variants
External Information: IL-12/IL-23 p40 Human antibody
| Google Scholar | Find more references for clone C8-6 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen IL-12/IL-23 p40 (Bioinformatic Harvester (EMBL)) |
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