Product details for  Ia (Chicken) antibody
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Ia (Chicken) antibody

Overview

Antigen: Ia
Antibody Type: Monoclonal
Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies-Blocking (FUNC-B)
Reactivity: Chicken, Bird (Avian)
Host: Mouse
Quantity: 0,5 mg
Price: 249,00 €   (Plus shipping costs and VAT)
Order number: ABIN135545
Availability: 7 to 10 days until shipment
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Additional Information:  Ia (Chicken) antibody

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Antigen Ia
Reactivity Chicken, Bird (Avian)
Antibody Type Monoclonal
Format Purified
Isotype IgM »Matching secondary antibodies
Description A 65 kDa glycoprotein consisting of a 32 kDa -chain non-covalently bound to a 27 kDa -chain The chicken Ia antigen is expressed on B cells, a subpopulation of the monocyte/macrophage lineage of cells, and mitogen-activated T cells. 1 The molecule recognized by this antibody is similar in structure to the murine Ia antigens and human DR antigens.
Clone CIa
Host Mouse

Application Details:  Ia (Chicken) antibody

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Application Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies-Blocking (FUNC-B)
Application Notes Working Dilutions: Flow Cytometry: Fluorescein (1 ug/10^6 cells), Biotin (1 /g106 cells), R-phycoerythrin (). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
Storage The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Restrictions For Research Use only

Publications:  Ia (Chicken) antibody

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Publications Ewert, Munchus, Chen et al.:
"Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal antibody to monomorphic determinants."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 132, Issue 5, pp. 2524-30, 1984/01 (PubMed)

External Information:  Ia (Chicken) antibody

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Google Scholar Find more references for clone CIa on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Ia (Bioinformatic Harvester (EMBL))