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Product details for JAB1 (Human) antibody
JAB1 (Human) antibody
Overview
| Antigen: | JAB1 |
| Antibody Type: | Polyclonal |
| Application: | Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN149295 |
| Availability: | 7 to 10 days until shipment |
Additional Information: JAB1 (Human) antibody
| Antigen | JAB1 |
| Immunogen | Synthetic peptide: SGSGMAQKTWELANNMQ with C-terminal added cysteine, conjugated to KLH, corresponding to amino acids 4-20 of Human JAB1. The corresponding sequence is identical in mouse and rat. |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Polyclonal |
| Isotype | IgG »Matching secondary antibodies |
| Description | Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported.JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the ce |
| Host | Rabbit |
Application Details: JAB1 (Human) antibody
| Application | Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | IHC-P: Use at a concentration of 10-20 ug/ml. This concentration is determined by indirect immunoperoxidase staining of formalin-fixed, paraffin-embedded sections of human breast carcinoma. IP: Use at an assay dependent dilution. 4.0-6.0 ug of the antibody immunoprecipitates J GTX1 from 0.5 mg of RIPA extract of rat PC12 cells. WB: Use at a concentration of 0.25-0.5 ug/ml, this concentration is determined using whole extracts of mouse NIH-3T3 cells. Predicted molecular weight: 38 kDa. Additional bands may be detected when blotting with some extract preparations. Detection of the J GTX1 band by immunoblotting is specifically inhibited with the immunizing peptide. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Buffer | Preservative: 15mM Sodium AzideConstituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Restrictions | For research use only |
External Information: JAB1 (Human) antibody
| Google Scholar | Find more references for antigen JAB1 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen JAB1 (Bioinformatic Harvester (EMBL)) |
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